The Combination of Mixed Lactic Acid Bacteria and Dietary Fiber Lowers Serum Cholesterol Levels and Fecal Harmful Enzyme Activities in Rats

다국어 초록 (Multilingual Abstract)

Probiotics such as lactic acid bacteria (LAB) and prebiotics such as fiber are generally considered beneficial for health. These affect the microflora composition and fermentation metabolites and consequently contribute to local and systemic effects in humans. The beneficial effects of probiotics can be improved when combined with prebiotics. Here we investigated the effects of a mixed LAB supplement combined with dietary fiber on the population of LAB in the gut, as well as on serum cholesterol levels, fecal water content and microbial harmful enzyme activities. For animal studies, 0.2 mL of mixed LAB (Bifidobacterium longum SPM1205, Lactobacillus acidophilus, and SAFELAC isolated from Pediococcus pentosaceus)supplement (10^7 ~ 10^8 colony forming units per day) was orally administered to male Sprague-Dawley rats every day for 2 weeks along with a diet containing 5% or 10% cellulose. The mixed LAB supplement combined with dietary cellulose significantly (p < 0.05) reduced serum total cholesterol and LDL levels. This combination also significantly (p < 0.05) increased the population of LAB and the fecal water content and significantly (p < 0.05) reduced microbial harmful enzyme (β-glucosidase, β-glucuronidase and tryptophanase) activities. These effects of LAB were particularly improved by its combination with 5% cellulose compared to the control (a diet without cellulose), and the 5% cellulose combination was more effective than the 10%cellulose combination. In conclusion, the incorporation of a fibrous diet such as cellulose with lactic acid bacteria improved the population of LAB, and daily consumption of this combination could reduce the serum cholesterol levels and activities of harmful enzymes such as β-glucosidase,β-glucuronidase, tryptophanase, urease in rats.

참고문헌 (Reference)

1 Kim, D. H., "pHinducible β-glucuronidase and β-glucosidase of intestinal bacteria" 40 : 1967-1969, 1992

2 Sartor, R. B., "Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases: antibiotics, probiotics, and prebiotics" 126 : 1620-1633, 2004

3 Gordon, D. T., "The importance of total dietary fiber in human nutrition and health" 25 : 75-76, 1992

4 Donohue, D. C., "Safety of probiotics" 15 : 563-569, 2006

5 Sung Sook Choi, "Safety Assessment of Potential Lactic Acid Bacteria Bifidobacterium longum SPM1205 Isolated from Healthy Koreans" 한국미생물학회 43 (43): 493-498, 2005

6 Blaut, M., "Relationship of prebiotics and food to intestinal microflora" 41 : I11-I16, 2002

7 Ouwehand, A. C., "Probiotics: mechanisms and established effects" 9 : 43-52, 1999

8 llins, M. D., "Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut" 69 : 1052S-1057S, 1999

9 Fuller, R., "Probiotics in man and animals" 66 : 365-378, 1989

10 Fooks, L. J., "Prebiotics, probiotics and human gut microbiology" 9 : 53-61, 1999

11 Reddy, B. S., "Nutritional factors and colon cancer" 35 : 175-190, 1995

12 Salminen, S., "Lactic acid bacteria, microbiology and functional aspects, in Lactic acid bacteria in health and disease" Marcel Dekker 211-253, 1998

13 Salminen, S., "Lactic acid bacteria in health and disease, in Lactic Acid Bacteria" Marcel Dekker 199-225, 1993

14 wan-kyu Lee, "Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use" 대한수의학회 5 (5): 391-395, 2004

15 Holzapfel, W. H., "Introduction to preand probiotics" 35 : 109-116, 2002

16 Lee, S.-M., "Inhibition effects of Lactic acid bacteria (LAB) on the Azoxymethane-induced colonic preneoplastic lesions" 38 : 169-175, 2000

17 Kim, D. H., "Induction and inhibition of indole of intestinal bacteria" 18 : 351-533, 1995

18 Gutmann, I., "In Methods of enzymatic analysis" Academic Press 1791-1794, 1974

19 Bengmark, S., "Immunonutrition: role of biosurfactants, fiber, and probiotic bacteria" 14 : 585-594, 1998

20 Fuller, R., "History and development of probiotics, in Probiotics-The Scientific Basis" Champman and Hall 1-18, 1992

21 Juskiewicz, J., "Effects of cellulose, carboxymethyl-cellulose and inulin fed to rats as single supplements or in combinations on their caecal parameters" 139 : 513-519, 2004

22 Bolognani, F., "Effect of lactobacilli, bifidobacteria and inulin on the formation of aberrant crypt foci in rats" 40 : 293-300, 2001

23 Marteau, P., "Effect of chronic ingestion of a fermented dairy product containing Lactobacillus acidophilus and Bifidobacterium bifidum on metabolic activities of the colonic microflora" 52 : 685-688, 1990

24 Gibson, G. R., "Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics" 125 : 1401-1412, 1995

25 Lim, C. C., "Dietary fibres as “prebiotics”: implications for colorectal cancer" 49 : 609-619, 2005

26 Ling, W. H., "Colonization and fecal enzyme activities after oral Lactobacillus GG administration in elderly nursing home residents" 36 : 162-166, 1992

27 Femia, A. P., "Antitumorigenic activity of the prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis on azoxymethane-induced colon carcinogenesis in rats" 23 : 1953-1960, 2002

28 Pool-Zobel, B. L., "Antigenotoxic properties of lactic acid bacteria in vivo in the gastrointestinal tract of rats" 20 : 271-281, 1993

29 Fernandes, C. F., "Anticarcinogenic and immunological properties of dietary lactobacilli" 53 : 704-710, 1990

30 Lee, D. K., "Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines" BioMed Central 8 (8): 0-0, 2008

31 Hudault, S., "Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection" 63 : 513-518, 1997

32 Fujimori, S., "A randomized controlled trial on the efficacy of symbiotic versus probiotic or prebiotic treatment to improve the quality of life in patients with ulcerative colitis" 25 : 520-525, 2009