Chicken serum uric acid level is regulated by glucose transporter 9

다국어 초록 (Multilingual Abstract)

Objective: Glucose transporter 9 (GLUT9) is a uric acid transporter that is associated with uric absorption in mice and humans; but it is unknown whether GLUT9 involves in chicken uric acid regulation. This experiment aimed to investigate the chicken GLUT9 expression and serum uric acid (SUA) level.
Methods: Sixty chickens were divided into 4 groups (n = 15): a control group (NC); a sulfonamide-treated group (SD) supplemented with sulfamonomethoxine sodium via drinking water (8 mg/L); a fishmeal group (FM) supplemented with 16% fishmeal in diet; and a uric acid-injection group (IU), where uric acid (250 mg/kg) was intraperitoneally injected once a day. The serum was collected weekly to detect the SUA level. Liver, kidney, jejunum, and ileum tissues were collected to detect the GLUT9 mRNA and protein expression.
Results: The results showed in the SD and IU groups, the SUA level increased and GLUT9 expression increased in the liver, but decreased in the kidney, jejunum, and ileum. In the FM group, the SUA level decreased slightly and GLUT9 expression increased in the kidney, but decreased in the liver, jejunum, and ileum. Correlation analysis revealed that liver GLUT9 expression correlated positively, and renal GLUT9 expression correlated negatively with the SUA level.
Conclusion: These results demonstrate that there may be a feedback regulation of GLUT9 in the chicken liver and kidney to maintain the SUA balance; however, the underlying mechanism needs to be investigated in future studies.

다국어 초록 (Multilingual Abstract)

Objective: Glucose transporter 9 (GLUT9) is a uric acid transporter that is associated with uric absorption in mice and humans; but it is unknown whether GLUT9 involves in chicken uric acid regulation. This experiment aimed to investigate the chicken GLUT9 expression and serum uric acid (SUA) level.Methods: Sixty chickens were divided into 4 groups (n = 15): a control group (NC); a sulfonamide-treated group (SD) supplemented with sulfamonomethoxine sodium via drinking water (8 mg/L); a fishmeal group (FM) supplemented with 16% fishmeal in diet; and a uric acid-injection group (IU), where uric acid (250 mg/kg) was intraperitoneally injected once a day. The serum was collected weekly to detect the SUA level. Liver, kidney, jejunum, and ileum tissues were collected to detect the GLUT9 mRNA and protein expression.Results: The results showed in the SD and IU groups, the SUA level increased and GLUT9 expression increased in the liver, but decreased in the kidney, jejunum, and ileum. In the FM group, the SUA level decreased slightly and GLUT9 expression increased in the kidney, but decreased in the liver, jejunum, and ileum. Correlation analysis revealed that liver GLUT9 expression correlated positively, and renal GLUT9 expression correlated negatively with the SUA level.Conclusion: These results demonstrate that there may be a feedback regulation of GLUT9 in the chicken liver and kidney to maintain the SUA balance; however, the underlying mechanism needs to be investigated in future studies.

참고문헌 (Reference)

1 Nagura M, "Uric acid metabolism of kidney and intestine in a rat model of chronic kidney disease" 35 : 550-558, 2016

2 Romi MM, "Uric acid causes kidney injury through inducing fibroblast expansion, Endothelin-1 expression, and inflammation" 18 : 326-, 2017

3 Keebaugh AC, "The evolutionary fate of the genes encoding the purine catabolic enzymes in hominoids, birds, and reptiles" 27 : 1359-1369, 2010

4 Mueckler M, "The SLC2(GLUT)family of membrane transporters" 34 : 121-138, 2013

5 Zhiqiang C, "Studies of determination of calcium, phosphonium and uric acid in urine by automatic biochemical analyzer" 6 : 461-463, 2006

6 Auberson M, "SLC2A9(GLUT9)mediates urate reabsorption in the mouse kidney" 470 : 1739-1751, 2018

7 Vitart V, "SLC2A9 is a newly identified urate transporter influencing serum urate concentration, urate excretion and gout" 40 : 437-442, 2008

8 Maesaka JK, "Regulation of renal urate excretion : a critical review" 32 : 917-933, 1998

9 Xu L, "Recent advances on uric acid transporters" 8 : 100852-100862, 2017

10 Zhang W, "Quantity of glucose transporter and appetite-associated factor mRNA in various tissues after insulin injection in chickens selected for low or high body weight" 45 : 1084-1094, 2013

11 Yamauchi T, "Primary hyperuricemia due to decreased renal uric acid excretion" 66 : 679-681, 2008

12 Anzai N, "Plasma urate level is directly regulated by a voltage-driven urate efflux transporter URATv1(SLC2A9)in humans" 283 : 26834-26838, 2008

13 El Ridi R, "Physiological functions and pathogenic potential of uric acid : a review" 8 : 487-493, 2017

14 Matsuo H, "Mutations in glucose transporter 9 gene SLC2A9 cause renal hypouricemia" 83 : 744-751, 2008

15 Keembiyehetty C, "Mouse glucose transporter 9 splice variants are expressed in adult liver and kidney and are up-regulated in diabetes" 20 : 686-697, 2006

16 Bibert S, "Mouse GLUT9 : evidences for a urate uniporter" 297 : F612-F619, 2009

17 Smith PK, "Measurement of protein using bicinchoninic acid" 150 : 76-85, 1985

18 Bai S, "Long-term effect of dietary overload lithium on the glucose metabolism in broiler chickens" 54 : 191-198, 2017

19 Kimura T, "Increased expression of SLC2A9 decreases urate excretion from the kidney" 30 : 1295-1301, 2011

20 Augustin R, "Identification and characterization of human glucose transporter-like protein-9(GLUT9) : alternative splicing alters trafficking" 279 : 16229-16236, 2004

21 Oka Y, "Hyperuricemia in hematologic malignancies is caused by an insufficient urinary excretion" 33 : 434-438, 2014

22 Ruiz A, "Human mutations in SLC2A9(Glut9)affect transport capacity for urate" 9 : 476-, 2018

23 Bu P, "Hormonal and chemical regulation of the Glut9 transporter in mice" 360 : 206-214, 2017

24 Preitner F, "Glut9 is a major regulator of urate homeostasis and its genetic inactivation induces hyperuricosuria and urate nephropathy" 106 : 15501-15506, 2009

25 Torres RJ, "GLUT9 influences uric acid concentration in patients with Lesch-Nyhan disease" 21 : 1270-1276, 2018

26 Clémençon B, "Expression, purification, and structural insights for the human uric acid transporter, GLUT9, using the Xenopus laevis oocytes system" 9 : e108852-, 2014

27 Mobasheri A, "Expression of the GLUT1 and GLUT9 facilitative glucose transporters in em-bryonic chondroblasts and mature chondrocytes in ovine articular cartilage" 29 : 249-260, 2005

28 Francisco JS, "Evaluation of the ImagePro Plus 4. 5 software for automatic counting of labeled nuclei by PCNA immunohistochemistry" 18 : 100-104, 2004

29 Mustafa S, "Effect of sulfa drugs on kidney function and renal scintigraphy" 19 : 210-216, 2014

30 DeBosch BJ, "Early-onset metabolic syndrome in mice lacking the intestinal uric acid transporter SLC2A9" 5 : 4642-, 2014

31 Field A, "Discovering statistics using IBM SPSS statistics" Sage Publications 2013

32 Liu Y, "Compound tufuling granules regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice" 21 : 823-829, 2015

33 Guo X, "Clinicopathology of gout in growing layers induced by high calcium and high protein diets" 46 : 641-646, 2005

34 Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method" 25 : 402-408, 2001

35 Ghoodjani A, "Advanced statistical methods and applications" Statistica 2018

36 Zeng Y, "Abcg2 deficiency augments oxidative stress and cognitive deficits in Tg-SwDI transgenic mice" 122 : 456-469, 2012