누드마우스에서 골 형성에 대한 BMP와 PDGF 복합사용의 효과 = Effect of the combined use of bone morphogenetic protein and platelet-derived growth factor on bone formation in nude mouse

다국어 초록 (Multilingual Abstract)

Bone morphogenetic protein(BMP) and platelet-derived growth factor(PDGF) have been demonstrated tostimulate bone formation when applied locally in vivo. To explore whether or not the combined use of BMP and PDGF could have promotive effect and synergic interaction on bone formation in vivo, bone marrow mesenchymal stem cells were treated with BMP-2, PDGF-BB, or BMP-2 plus PDGF-BB, and then these cells were injected into the subcutaneous space on the dorsum of nude mice. The bone formation was evaluated after 12 weeks. Histomorphometric analysis demonstrated that the subcutaneous nodules formed in nude mice contained 25.3% newly formed bone in the BMP-2 treated cells, 14.4% newly formed bone in the PDGF-BB treated cells, and 8.9% newly formed bone in the RMP-2 plus PDGF-BB treated cells. The results showed that the combination of BMP-2 and PDGF-BB had neither a promotive effect nor synergic interact on bone formation in vivo.

참고문헌 (Reference)

1 "and myofibroblasts in excisional wounds" 629-646, 1991

2 "and PDGFBB stimulate chemotactic migration of primary human mesenchymal progenitor cells" 305-2 4 312,

3 "Type beta transforming growth factor:a bifunctional regulator of cellular growth" 82 : 119-123, 1985

4 "Transforming growth factor beta:biological function and chemical structure" 233 : 532-534, 1986

5 "The effects of systemically administered PDGF-BB on the rodent skeleton" 11 : 238-247, 1996

6 "The bone regenerative effect of plateletderived growth factorBB delivered with a chitosan/tricalcium phosphate sponge carrier" 71 : 418-424, 2000

7 "Role of platelet-derived growth factor in wound healing:synergistic effects with other growth factors" 84 : 7696-7700, 1987

8 "Recombinant human bone morphogenetic protein-2 potentiates the in vivo osteogenic ability of marrow/hydroxyapatite composit- es" 25 : 201-208, 2001

9 "Poly(a-hydroxy acids)carriers for bone morphogenetic proteins" 17 : 187-194, 1996

10 "Osteogenesis in marrow-derived mesenchymal stem cells/porous ceramic composites transplanted subcutaneously:effect fibronectin and laminin on cell retention and rate of osteogenesis expression" 1 : 23-32, 1992

11 "Osteochondrogenic potential of marrow mesenchymal progenitor cells exposed to TGF-beta 1 or PDGF-BB as assayed in vivo and in vitro" 11 : 1264-1273, 1996

12 "Human osteoblasts synthesize and respond to platelet-derived growth fac- tor" 261 : 1991

13 "Human osteoblasts synthesize and respond to platelet-derived growth fac- tor" 261 : 1991

14 "Growth factors in wound healing" 84 : 640-646, 1989

15 "Expressions of bone morphogenetic proteins in fracture healing" 355 : 1998

16 "Experimental study on bone formation potential of cryopreserved human bone marrow mesenchymal cell/hydroxyapatite complex in the presence of recombinant human bone morphogenetic protein-2" 14 : 108-116, 2003

17 "Effects of platelet-derived growth factor on bone formation in vitro" 140 : 530-537, 1989

18 "Effect of platelet-derived growth factor-BB on bone formation in calvarial defects:an experimental study in rabbits" 105 : 59-66, 1997

19 "Ecto- pic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate" 108 : 434-443, 2001

20 "Dis- tinct and overlapping patterns of localization of bone morphogenetic protein(BMP)family members and a bmp type II receptor during fracture healing in rats" 22 : 605-612, 1998

21 "Bone morphogenetic proteins:an update on basic biology and clinical relevance" 17 : 269-278, 1999

22 "Bone formation in vitro by stromal cells obtained from marrow of young adults" 254 : 317-330, 1988