Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains|RISS 상세보기

다국어 초록 (Multilingual Abstract)

The aims of this study is to compare the growth and glucose metabolism of three Lactobacillus reuteri strains (i.e.
DSM 20016, DSM 17938, and ATCC 53608) which are lactic acid bacteria of interest used for diverse applications such as probiotics implying the production of biomass, or for the production of valuable chemicals (3-hydroxypropionaldehyde, 3-hydroxypropionic acid, 1,3-propanediol). However, the physiological diversity inside the species, even for basic metabolisms, like its capacity of acidification or glucose metabolism, has not been studied yet. In the present work, the growth and metabolism of three strains representative of the species diversity have been studied in batch mode. The strains were compared through characterization of growth kinetics and evaluation of acidification kinetics, substrate consumption and product formation. The results showed significant differences between the three strains which may be explained, at least in part, by variations in the distribution of carbon source between two glycolytic pathways during the bacterial growth: the phosphoketolase or heterolactic pathway (PKP) and the Embden-Meyerhof pathway (EMP). It was also shown that, in the context of obtaining a large amount of biomass, DSM 20016 and DSM 17938 strains were the most effective in terms of growth kinetics. The DSM 17938 strain, which shows the more significant metabolic shift from EMP to PKP when the pH decreases, is more effective for lactate production.

번역하기

참고문헌 (Reference)

1 Casas, I.A., "Validation of probiotic concept: Lactobacillus reuteri confers broad-spectrum protection against disease in humans and animals" 12 : 247-285, 2000

2 Talarico, T.L., "Utilization of glycerol as a hydrogen acceptor by Lactobacillus reuteri: purification of 1.3 propanediol: NAD+oxidoreductase" 56 : 1195-1197, 1990

3 Pieterse, B., "Unravelling the multiple effects of lactic acid stress on Lactobacillus plantarum by transcription profiling" 151 : 3881-3894, 2005

4 van Niel, E.W.J., "The potential of biodetoxification activity as a probiotic property of Lactobacillus reuteri. Internat" 152 : 206-210, 2012

5 Ahrné, S., "The normal Lactobacillus flora of healthy human rectal and oral mucosa" 85 : 88-94, 1998

6 Frese, S.A., "The evolution of host specialization in the vertebrate gut symbiont Lactobacillus reuteri" 7 : e1001314-, 2011

7 Werner, S., "Revisiting the thermodynamic theory of optimal ATP stoichiometries by analysis of various ATP-producing metabolic pathways" 71 : 346-355, 2010

8 Rosander, A., "Removal of antibiotic resistance gene-carrying plasmids from Lactobacillus reuteri ATCC 55730 and characterization of the resulting daughter strain, L. reuteri DSM 17938" 74 : 6032-6040, 2008

9 Garrigues, C., "Regulation of pyruvate metabolism in Lactococcus lactis depends on the imbalance between catabolism and anabolism" 74 : 108-115, 2001

10 Vollenweider, S., "Purification and structural characterization of 3-hydroxypropionaldehyde and its derivatives" 51 : 3287-3293, 2003