Isolation of Biflavonoids from Endemic Plants and their Anti-inflammatory Activities

다국어 초록 (Multilingual Abstract)

Inflammation is the defensive response of the organism to infection by infectious agents and removal of damaged tissues. Inflammation has acute inflammation and chronic inflammation. Inflammatory bowel diseases (IBD) primarily, ulcerative colitis and Crohn’s disease is characterized by chronic inflammatory disease.
Biflavonoid have known to exhibit an anti-inflammatory effect. For the treatment of inflammatory diseases, we searched for biflavonoids that exhibit antiinflammatory activity in endemic plants. We isolated robustaflavone (R), amentoflavone (A) from Nandina domestica, amentoflavone (A), 7,7''-di-Omethyl amentoflavone (mA), hinokiflavone (H) and 7''-O-methyl methylhinokiflavone (mH) from Selaginella tamaricsina, and 7,4',7''-tri-O-methyl amentoflavone (tA) from Podocarpus macrophyllus. It investigated their antiinflammatory activities in lipopolysaccharide (LPS)-mediated murine macrophages.
Compounds had nitric oxide (NO) inhibitory effect without cytotoxicity.
Among them, two strong anti-inflammatory compounds, H and mH from Selaginella tamariscina were examined their anti-inflammatory activities in LPSmediated murine macrophages and colon epithelial cells. H and mH suppressed the production of the inflammatory mediators, NO, interleukin (IL)-6, IL-8 and tumor-necrosis factor (TNF)-α, which are most highly activated in IBD. In addition, western blot analysis revealed that H and mH suppressed the LPSinduced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and the activation of nuclear factor-κB (NF-κB) and extracellular regulated kinases (ERK) 1/2. These results suggest that H and mH are compounds having potent anti-inflammatory effects that could be used to treat such diseases as IBD.

목차 (Table of Contents)

• Ⅰ. Introduction . 1
• Ⅱ. Materials and Methods. 14
• 1. Reagent and instrument 14
• 2. Plant 17
• 3. Evaluation of anti-inflammatory activity . 25
• Ⅲ. Result and discussion . 28
• 1. Identification of chemical structure of compounds isolated from N. domestica 28
• 2. Biflavonoids from S. tamariscina 35
• 3. Biflavonoids from P. macrophyllus . 36
• 4. Biflavonoids from endemic plants 37
• 5. Anti-inflammatory activities of compounds isolated from endemic plants 38
• 5. Discussion 54
• Ⅳ. Conclusion . 57
• Ⅴ. Reference . 58
• 국문초록 63