The events in the middle multiplication sequences (10 to 18 hours postinfection) of Autographa californica nuclear polyhedrosis virus (AcNPV) ts-B1074) in Spodoptera frugiperda cells at 25℃ and 32.5℃ were comparatively visualized by electron micro...
The events in the middle multiplication sequences (10 to 18 hours postinfection) of Autographa californica nuclear polyhedrosis virus (AcNPV) ts-B1074) in Spodoptera frugiperda cells at 25℃ and 32.5℃ were comparatively visualized by electron microscopy. Early stages (0 time to 8 hours postinfection) of the AcNPV L-1 and ts-B1074 multiplication appeared to be the same pathways at both temperatures (Oh and Lee, 1987). The general aspects of the middle events in the multiplication were (1) the formations of the virogenic stroma, progeny nucleocapsid and prepolyhedra occlusion bodies, (2) bundling, enveloping and budding of the nucleocapsids, and (3) the hypertrophy of the nuclear membrane. The middle stages in the multiplication of the ts-B1074 at 25℃ and 32.5℃ were slightly different in the prepolyhedra, virogenic stroma and nucleocapsid formations at 10 h to 18 h postinfections which were stimulated and appeared early at 32.5℃. Amounts of nucleocapsid production and bundling and cytopathic effects not affected by temperatures. The amounts of extracelluar nonoccluded nucleocapsids were greater at 25℃.