수종 담수적조 원인종들의 형광특성과 적용연구 = The study on the Fluorescence Characteristics of Several Freshwater Bloom Forming Algal Species and Its Application

다국어 초록 (Multilingual Abstract)

The freshwater blooms mainly blue-green algal blooms occur frequently in the lower Naktong River in summer, which provoke many socio-economical problems; therefore, the early detection of bloom events are demanding through the quantitative and qualitative analyses of blue green algal species. The in vivo fluorescence properties of cultured strains of Microcystis aeruginosa, M. viridis, M. wesenbergii, M. ichthyoblabe, Anabaena cylindrica, A. flos-aquae, and Synedra sp. were investigated. Wild phytoplankton communities of the lower Naktong River were also monitored at four stations in terms of their standing stocks, biomass and fluorescence properties compared with its absorption spectram. The 77K fluorescence emission spectra of each cultured strains normalized at 620 nm was very specific and enabled to detect of blue green algal biomass qualitatively and quantitatively. The relative chlorophyll a concentration determined by chlorophyll fluorescence analysis method showed significant relationship with chlorophyll a concentration determined by solvent extraction method ($R^2$ = 0.906), and the blue-green algal cell number determined by microscopic observation ($R^2$ = 0.588), which gives insight into applications to early detection of blue green algal bloom.

참고문헌 (Reference)

1 "제4권 생화학 총설집" 265-271 엽록소에서방출되는형광의분석을통한광합성연구

2 "the perception of water quality and the prioritisation of eutrophication control" cya (cya): 51-60, 2000

3 "sensitivity to a wide range of toxicants" lim (lim): 275-284,

4 "photosynthetic pigments in vitro Light emission by plants and bacteria" New York Academic Press 99-133,

5 "and Connolly J" 1986 r

6 "a taxonomic tool for studying the structure of phytoplankton populations" 617-632, 1985

7 "a potential tool for rapid detection and determination of herbicide phytotoxicity in algae" 20031983-1990

8 "The molecular biology of cyanobacteria" Kluwer Academic press 1994

9 "Survival of marine phytoplankton in high concentration of heavy metals and uptake of copper" 249-264, 1977

10 "Survey of algal species" 1978

11 "State transition in a phycobilisome-less mutant of the cyanobacterium Synechococcus sp" 66-7002 974 73, 1989

12 "Simple procedure for growing SCOR reference microalgal cultures Phytoplankton pigments in oceanography" UNESCO Publ 1997181-205

13 "Selection and characterization of transposon tagging mutants of Synechocystis sp PCC 6803 sensitive to high-light and oxidative stresses" 47 : 289-299, 2004

14 "Release of geosmin Anabaena circinalis following treatment with aluminium sulphate" 31 : 187-194, 1995187-194

15 "Quantitative analysis of 77K fluorescence emission spectra in Synechocystis sp PCC 6714 and Chlamydomanas reinhardtii with variable PSⅠ/PSⅡ stoichiometries" 53 : 141-148, 1997

16 "Phycobilisome core mutants of Synechosystis PCC 6803" ver dimag (ver dimag): -1231, 1995189-196

17 "New spectrometric equations for determining chlorophyll a" 1975191-194

18 "Mechanism of toxicity of ionic copper and copper complexes to algae" 511-519, 1987

19 "Illustrations of the freshwater plankton of Japan" 1977

20 "Fluorometric determination of chlorophyll" 3-15, 1965

21 "Excitation energy transfer from phycobilisomes to photosystems a phenomenon associated with the temporal separation of photosynthesis and nitrogen fixation in a cyanobacterium Plectonema boryamum" 139-1459 147, 2000

22 "Evaluation of different algal species sensitivity to mercury and metolachlor by PAM-fluorometry" 45 : 589-598, 2001

23 "Ecologia del fitoplancon in Limnologia" 247-330, 1983

24 "Disease related to freshwater bluegreen algae toxins and control measures Algal toxins in seafood and drinking water" Cambridge Academic Press 1993187-209

25 "Bioactive compounds produced by cyanobacteria" 17 : 373-384, 1996

26 "Application of pigment methods to" 1997

27 "Application of chlorophyll fluorescence" Kluwer Academic Press 1988

28 "Absorption and fluorescence emission by intact cells Light emission by plants and bacteria" chloroplasts and chlorophyllprotein complexes New York Academic Press 137-159, 1986