PCR based Detection of Helicobacter spp. in Saliva, Dental plaque, Vomitus and Feces of Dogs

다국어 초록 (Multilingual Abstract)

This study was aimed to determine the prevalence of Helicobacter spp. in privately owned pet dog’s oral
cavity samples (saliva, dental plaque, vomitus) and fecal samples in Korea and to evaluate the potential route for
transmission. Total 100 patients dogs attending one Veterinary Medical Teaching Hospital were examined by
Helicobacter genus-specific PCR assay and these dogs were divided into two groups whether they had gastrointestinal
signs (vomiting, nausea and diarrhea) or not. The total detection rate of Helicobacter spp. by PCR in saliva, dental
plaque and fecal samples was 23%, 1% and 68% respectively. The difference of prevalence with regarding the
gastrointestinal sings was not significant. In vomitus, two of seven samples had positive results. These results suggested
that Helicobacter spp. are present in the oral cavity although they were present in very low number and are not like
to be normal oral flora of the oral cavity and Helicobacter spp. in dogs could be transmitted through oral-oral, gastrooral
and fecal-oral route.

참고문헌 (Reference)

1 Weber AF, "Some observations concerning the presence of spirilla in the fundic glands of dogs and cats" 19 : 677-680, 1958

2 Lee A, "Role of Helicobacter felis on chronic canine gastritis" 29 : 487-494, 1992

3 Allaker RP, "Prevalence of Helicobacter pylori at oral and gastrointestinal sites in children Evidence for possible oral-to-oral transmission" 51 : 312-317, 2002

4 Eaton KA, "Prevalence and varieties of Helicobacter species in dogs from random sources and pet dogs: Animal and public health implications" 34 : 3165-3170, 1996

5 Hong-Ryul Han, "Prevalence and Clinical Characterization of Gastric Helicobacter Species Infection of Dogs and Cats in Korea" 대한수의학회 3 (3): 123-134, 2002

6 Dieterich C, "Presence of multiple “Helicobacter heilmannii” strains in an individual suffering from ulcers and in his two cats" 36 : 1366-1370, 1998

7 Geyer C, "Occurrence of spiral-shaped bacteria in gastric biopsies of dogs and cats" 133 : 18-19, 1993

8 Happonen I, "Occurrence and topographical mapping of gastric Helicobacter-like organisms and their association with histological changes in apparently healthy dogs and cats" 43 : 305-315, 1996

9 Thomas JE, "Isolation of Helicobacter pylori from human faeces" 2 : 1194-1195, 1992

10 Harper CM, "Isolation and characterization of Helicobacter sp. from the gastric mucosa of dolphins, lagenorhynchus actus and delphinus delphis" 66 : 4751-4757, 2000

11 Riggio MP, "Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients" 48 : 317-322, 1999

12 Umeda M, "High prevalence of Helicobacter pylori detected by PCR in the oral cavities of periodontitis patients" 74 : 129-134, 2003

13 Hermanns W, "Helicobacter- like organisms: Histological examination of gastric biopsies from dogs and cats" 112 : 307-318, 1995

14 Fox JG, "Helicobacter pylori-induced gastritis in the domestic cat" 63 : 2674-2680, 1995

15 Handt LK, "Helicobacter pylori isolated from domestic cat: public health implications" 62 : 2367-2374, 1994

16 Parsonnet J, "Helicobacter pylori infection and the risk of gastric calcinoma" 325 : 1127-1231, 1991

17 Uemura N, "Helicobacter pylori infection and the development of cancer" 345 : 784-789, 2001

18 Bronsdon MA, "Helicobacter nemestrinae sp. Nov., a spiral bacterium found in the stomach of a pig tailed macaque (Macaca nemestrina)" 41 : 148-153, 1991

19 Stolte M, "Helicobacter heilmannii ( formerly Gastrospirillum hominis ) gastritis : an infection transmitted by animals?" 29 : 1061-1064, 1994

20 Eaton KA, "Helicobacter acnonyx sp. Nov., isolated from cheetahs with gastritis" 43 : 99-106, 1993

21 Heilmann KL, "Gastritis due to spiral shaped bacteria other than H. pylori: clinical, hostological, and ultrastructural findings" 32 : 137-140, 1991

22 Shames B, "Evidence for the occurrence of the same strain of Campylobacter pylori in the stomach and dental plaque" 27 : 2849-2850, 1989

23 Goosen G, "Evaluation of novel heminested PCR assay based on the phosphoglucosamine mutase gene for detection of Helico-bacter pylori in saliva and dental plaque" 40 : 205-209, 2002

24 Cheol-YongHwang, "Development of Non-Invasive Fecal PCR Assay for Detecting the Helicobacter Species Infection in Dogs" 한국임상수의학회 19 (19): 295-298, 2002

25 Alejandra B, "Detection of Helicobacter pylori DNA in the oral cavity and gastroduodenal system of a Venezuelan population" 51 : 764-770, 2002

26 Yamasaki K, "Comparison of gastric lesion in dogs and cats with and without gastric spiral organism" 212 : 529-533, 1998

27 Megenegatti M, "Clinical significance of Helicobacter pylori seropositivity and seronegativity in asymptomatic blood donors" 43 : 2542-2548, 1998

28 Tomson MA, "Cleghorn GJ. Caninehuman transmission of Gastrospillium hominis" 343 : 1605-1607, 1994

29 Fox JG, "Campylobacter pylori subs. Mustelae sub. Nov. isolated from the gastric mucosa of ferrets (Mustela putorious furo), and an emended description of Campylobacter pylori" 38 : 367-370, 1988

30 Queiroz DMM, "Association between Helico-bacter and gastric ulcers disease of pars esophagea in swine" 111 : 19-27, 1996

31 Lavelle JP, "Acute gastritis associated with spiral organisms from cats" 39 : 744-750, 1994

32 Chuanfu LI, "A newly developed PCR assay of H. pylori in gastric biopsy, saliva, and feces; Evidence of high prevalence of H. pylori in saliva supports oral transmission" 41 : 2142-2149, 1996