흰쥐의 간손상(肝損傷)에 대한 가미유간탕(加味愈肝湯)의 간(肝) 보호효과(保護效果) = Protective Effects of Gami Yugan-tang on Liver Damage in Rats

다국어 초록 (Multilingual Abstract)

In order to investigate the protective effects of Gami Yugan-tang on liver damage in rats induced by $CCl_{4}$ and d-galactosamine, the serum transaminase(ALT & AST), alkaline phosphatase(ALP), lactic dehydrogenase(LDH), superoxide dismutase(SOD), catalase, glutathione S-transferase(GST), glutathione peroxidase(GPX) for enzyme activities, and lipid peroxidation were measured. All animals were divided into 4 groups: normal group (untreated), control group (treated with 0.9% saline solution), sample I group (treated with 740mg/kg Gami Yugan-tang), and sample II group (treated with 1,480mg/kg Gami Yugan-tang). The results were as follows : 1. The results of liver damage in rats induced by $CCl_4$ : The protective effects of ALT were displayed in sample I and sample II, and AST, ALP, LDH, SOD, catalase, GST, GPX, and lipid peroxidation were noted in sample II group. It showed slight necrosis of hepatic cell and pathologic changes, for example, inflammatory cells infiltration were improved in sample II group compared to the control group. 2. The results of liver damage in rats induced by d-galactosamine : The inhibitory effects of AST, ALT, LDH, and ALP activities were noted in both sample I and sample II groups. The findings from this experiment suggests that Gami Yugan-tang has protective effects against liver damage in rats induced by $CCl_{4}$ and d-galactosamine.

참고문헌 (Reference)

1 임화경, "예덕나무피엑스의 사염화탄소 및 갈락토사민 유발 간독성에 대한 보호 및 치료효과" 7 : 35-43, 1999

2 "식품의약품안전청고시 제 1999-61호 의약품 등의 독성시험기준, 식품의약품안전청(1999. 12. 22 제정)"

3 서울대학교 의과대학, "소화기학" 서울대학교출판부 357-359, 1991

4 권칠용, "소시호탕합보중익기탕과 기국지황탕이 Dimethylnitrosamine 및 담관결찰로 유발된 백서의 간기능섬유화에 미치는 영향" 9 : 115-121, 1994

5 김안근, "산화적 스트레스 및 항산화제가 항산화효소 활성에 미치는 영향" 9 : 249-257, 2001

6 이종훈, "茵蔯분획물이 간세포활성, 세포주기 및 Fas mediated Apoptosis에 미치는 영향" 21 (21): 363-368, 2000

7 김건진, "茵蔯蒿湯加雄膽의 經口投與가 CCl4 投與로 유발된 肝硬變에 미치는 영향" 9 (9): 231-249, 2001

8 강경태, "茵蔯淸肝湯加味方이 간조직의 섬유화 억제에 미치는 영향" 대한한의학회 23 (23): 227-244, 2002

9 周禎祥, "肝膽病效方230首" 科學技術文獻出版社 53-, 1995

10 全國韓醫科大學 肝系內科學敎授, "肝系內科學" 東洋醫學硏究院 24-38, 1992

11 곽경규, "生肝健脾湯을 이용한 慢性肝炎 35例에 대한 臨床分析" 6 (6): 313-318, 1997

12 全國韓醫科大學 本草學敎授, "本草學" 永林社 149-, 1991

13 孫明輝, "愈肝方治療慢性乙型肝炎臨床觀察" 9 : 389-, 1989

14 陳貴廷, "實用中西醫結合診斷治療學" 中國醫藥科技出版社 169-170, 1991

15 손창규, "加味淸肝湯의 肝保護 및 免疫調節效果" 23 (23): 28-38, 2002

16 Lisa A. Brennan, "The effect of vitamin C or vitamin E supplementation on basal and H2O2-induced DNA damage in human lymphocytes" 84 : 195-202, 2000

17 Keppler, D, "Studies on the mechanism of galactosamine hepatitis Accumulation of galactosamine-1-phosphate and tis inhibition of UDP-glucose pyrophosphorylase" 10 : 219-225, 1996

18 Oyanagui, Y., "Reevaluation of assay methods and establishment of kit for superoxide dismutase activity" 42 : 290-296, 1948

19 Hayes, "Principles and Method of Toxicology" Raben Press 407-445, 1982

20 S. Takeda, "Pharmacological studies on schizandra fruits. III. Effects of wuweizisu C, a lignan component of schizandra fruits, on experimental liver injuries in rats" 85 : 193-, 1985

21 S. Maeda, "Pharmacological studies on Schzandra Fruits. II." 102 (102): 579-, 1982

22 Y. Niiho, "Pharmacological Studies on Puerariae Flos. II. The effects of Puerariae Flos on Alcohol-Induced Unusual Metabolism and Experimental Liver injury in Mice" 110 : 604-, 1990

23 McCay, P.B., "Oxygen and Carbon-centered free radical formation during carbon tetrachloride metabolism" 259 : 2135-2143, 1984

24 Wroblewski, F, "Lactic dehydrogenase activity in blood" 90 : 210-, 1955

25 Marklund, S, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase" 47 : 469-474, 1974

26 유희정, "IMF에 관련된 경제적 스트레스가 정신 건강에 미치는 영향" 39 (39): 65-77, 2000

27 박영신, "IMF 시대가 한국인의 스트레스에 미친 영향" 4 (4): 57-79, 1999

28 Habig, W. H., "Glutathion S-tansferase" 249 : 7130-, 1974

29 Kind, P.R.N, "Estimation plasma phosphatase by determination of hydrolysed phenol with aminoantipyrine" 7 : 322-, 1954

30 Susan Shull, "Differential regulation of antioxidant enzymes in response to oxidants" 266 : 24398-24403, 1991

31 Abei, H., "Catalase in vitro" 105 : 93-, 1984

32 임화경, "Bergenin 및 acetylbergenin의 사염화탄소 유발 간독성에 대한 치료효과 I" 8 : 293-298, 2000

33 Flohe, L, "Assays of glutathione peroxidase" 105 : 114-121, 184

34 Chance, B, "Assay of catalase and peroxidase" Academic Press 764-775, 1955

35 Yokozawa, T, "Antioxidation activity of Wen-Pi-Tang in vitro" 50 (50): 243-, 1996

36 Reitman, S, "A colorimetric method for the determination of serum glutamic oxaloacetic acid and glutamic pyruvic transaminase" 28 : 56-, 1957