X線 全身照射에 依한 家兎蟲垂 淋巴組織의 微細構造의 變化 = Ultrastructural Changes of the Appendiceal Lymphoid Tissues of the Rabbit Induced by X-irradiation

국문 초록 (Abstract)

成熱家兎를 實驗動物로 하여 600γ의 X線을 單回 全身照射하고 B淋巴球系의 中樞淋巴組織으로 알려진 충수임파조직에 惹起되는 變化를 機能的 組織構造에 따라 病理組織學的 및 電子顯微鏡的으로 檢索하여 다음과 같은 結果를 얻었다.
組織學的으로는 충수임파조직의 dome과 follicle에서 大小淋巴球의 甚한 懷死 消失과 大食細胞의 顯著한 增加를 볼 수 있었으나, 胸腺依存域인 TDA에서는 淋巴球의 壞死 消失이 比較的 輕한 程度로 認定되었다. 上記한 變化들은 X線 照射後 3時間에도 顯著하였으나 照射後 1日에는 더욱 甚하였다.
電子顯微鏡的으로는, X線 照射後 3時間에는 dome 및 follicle에서 淋巴球의 核染色質은 核邊緣部로 凝集되고 核中心部는 稀薄化되었고 어떤 淋巴球에서는 核濃縮을 일으키고 있었으며, mitochondria에서는 cristae가 消失되고 空胞化하는 傾向이 있었다. 그리고 組織球가 增加하고 그 細胞質內에서 lysosome의 增加, 濃縮된 核斷片의 탐식 等의 所見도 輕度로 認定되었다. 照射後 1日에는 dome 및 follicle에서 淋巴球는 大多數가 破壞消失되었고, 組織球가 顯著하게 增加되어 破壞된 細胞成分들을 활발하게 탐식하고 있는 것을 볼 수 있었다. 그러나 TDA에서는 淋巴球에서 染色質의 核邊緣部 凝集 乃至 核濃縮 그리고 mitochondria의 變性을 多少 볼 수 있었으나 甚한 破壞性變化는 認定되지 않았으며, 組織球의 貪　機能도 顯著하지 않았다.
以上의 實驗成績으로 보아, X線 照射에 對해서 B淋巴球를 生産하는 中樞淋巴組織의 淋巴球들은 感受性이 높아서 早期에 甚하게 破壞되며, 抹消淋巴組織에 分布하는 T淋巴球는 比較的 抵抗性이 있어서 損傷을 적게 받는 것으로 推定된다.

다국어 초록 (Multilingual Abstract)

This study was undertaken to investigate the structural changes of the appendiceal lymphoid tissues of the adult rabbit after whole body X-irradiation by the light and electron microscopes.
Nine healthy rabbits, weighing 2 to 2.5 Kgm were divided into 2 groups:
Group 1: Control group: Untreated (3 rabbits)
Group 2. Experimental group: A single air dose of 600r whole body X-irradiation was given (6 rabbits). Experimental animals were sacrificed at 3 hours and 1 day after irradiation and examined light and electron microscopically.
The results were summarized as follows:
Light microscopically, the appendical lymphoid tissues showed marked necrotic loss of small and large lymphocytes as well as considerable increase of macrophages in the dome, corona and follicle. But thymus dependent area (TDA) was relatively well preserved with mild degree's necrotic loss of the cells. These changes were apparent 3 hours after irradiation and more prominent after 1 day.
Electron microscopically, the nuclei of lymphocytes in the dome and follicle revealed peripheral aggregation of chromatin with central rarefaction after 3 hours. Some lymphocytes showed pyknotic changes. Mitochondria of the these cells were vacuolated and lossed their cristae.
Macrophages at this time were increased in number and showed increase of lysosomes with phagocytosis of nuclear fragments. After 1 day, lymphocytes in the dome and follicle were substantially destructed and lossed. Macrophages were markedly increased in number and showed considerable number of phagosomes containing destructed cellular debris in the cytoplasms. In the TDA, only milder degree of chromatin aggregation, pyknosis and mitochondrial degeneration were found but apparent destructive changes were not impresive. Phagocytic activities of macrophages were also inapparent.
These results suggested that central lymphoid tissue for differentiation of B-lymphocytes was very sensitive to X-irradiation and severely affected at early stage, while T-lymphocytes distributed to peripheral lymphoid tissue were relatively radioresistant and affected lesser degree.