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      Kanamycin Acetyltransferase Gene from Kanamycin-producing Streptomyces kanamyceticus IFO 13414

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      https://www.riss.kr/link?id=E688983

      • 저자
      • 발행기관
      • 발행연도

        1998년

      • 작성언어

        English

      • KDC

        510.000

      • 자료형태

        한국연구재단(NRF)

      • 수록면

        1-8

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      다국어 초록 (Multilingual Abstract)

      A kanamycin producer, Streptomyces kanamyceticus IFO 13414 is highly resistant to kanamycin Cloning of the kanamucin resistance genes in S lividans 1326 with pIJ702 gave several kanamycin resistant transformants Two transformants, S lividans SNUS 90041 and S.lividans SNUS 91051 showed similar resistance patterns to various aminoglycoside antibiotics Gene mapping experiments revealed that plasmids pSJ5030 and pSJ2131 isolated from the transformants have common resistant gene fragments Subcloning of pSJ5030 gave a 18 Kb gene fragment which showed resistance to kanamucin Cell free extracts of S lividans SNUS 90041, S lividans SNUS 91051 and subclone a S lividans SNUS 91064 showed kanamycin acetyltransferase activity The detailed gene map is included
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      A kanamycin producer, Streptomyces kanamyceticus IFO 13414 is highly resistant to kanamycin Cloning of the kanamucin resistance genes in S lividans 1326 with pIJ702 gave several kanamycin resistant transformants Two transformants, S lividans SNUS 9004...

      A kanamycin producer, Streptomyces kanamyceticus IFO 13414 is highly resistant to kanamycin Cloning of the kanamucin resistance genes in S lividans 1326 with pIJ702 gave several kanamycin resistant transformants Two transformants, S lividans SNUS 90041 and S.lividans SNUS 91051 showed similar resistance patterns to various aminoglycoside antibiotics Gene mapping experiments revealed that plasmids pSJ5030 and pSJ2131 isolated from the transformants have common resistant gene fragments Subcloning of pSJ5030 gave a 18 Kb gene fragment which showed resistance to kanamucin Cell free extracts of S lividans SNUS 90041, S lividans SNUS 91051 and subclone a S lividans SNUS 91064 showed kanamycin acetyltransferase activity The detailed gene map is included

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      목차 (Table of Contents)

      • INTRODUCTION
      • MATERIALS AND METHODS
      • RESULTS
      • DISCUSSION
      • REFERENCES CITED
      • INTRODUCTION
      • MATERIALS AND METHODS
      • RESULTS
      • DISCUSSION
      • REFERENCES CITED
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