기니 피그에서 콜레시스토키닌 투여가 고탄수화물 , 저지방 , 저단백질식에 의한 색소성 담석의 형성에 미치는 영향 = Effect of Cholecystokinin on the Formation of Pigment Gallstone by High Carcohydrate, Low Fat and Low Protein Diet in Guinea Pig기니 피그에서 콜레시스토키닌 투여가 고탄수화물 , 저지방 , 저단백질식에 의한 색소성 담석의 형성에 미치는 영향

다국어 초록 (Multilingual Abstract)

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다국어 초록 (Multilingual Abstract)

W e reported an animal model of pigment gallstone induced by carbohydrate-rich diet main- ly composed with rice. However, the machanisms have not been clearly understood. Under the hypothesis that high-carbohydrate diet (CHO) might induce gallstone formation via the mechanism of the relative bile stasis caused by low secretion of cholecystokin (CCK), the aim of this study was to examine whether exogenous CCK administration inhibits gall- stone formation induced by CHO in guinea pig. Male guinea pigs were divided into 3 groups(Group 1: control chow-fed group, Group 2: high carbohydrate fed group, Group 3: high cabohydrate fed and CCK injected group). High carbo- hydrate diet was 63.2% carbohydrate(45.8% in control chow), mainly composed of rice, and Group 3 received a daily injection of cholecystokinin(0.5nmol/kg). After 6 weeks of feeding, the guinea pigs were sacrificed. The stones were analyzed by infrared spectrophotornetry and gallbladder bile was analyzed by using commercial kits. In group 1, gallstone was found in one case out of 10 animals, in Group 2, 9 out of 14(p<0.05 vs Group 1) and in Group 3, 3 out of 12(p<0 05 vs Group 2). There were no differences in the concentrations of total calcium, total bilirubin, cholesterol, phospholipid and bile acid of gall- bladder bile among 3 groups. The stones were mainly composed of calcium bilirubinate, cho- lesterol, calcium phosphate, and calcium palmitate, which were similar to human calcium bilirubinate stone. The prevalence of stone formation was lowered with administration of CCK in CHO fed animals. A possible mechanism is that exogenous CCK may recover the low CCK release by CHO. It is suggested that bile stasis caused by poor CCK release which may be due to CHO be the one of the contributing factors in the formation of pigment stones in guinea pigs. (Korean J Gastroenterol 1 994; 26: 151 156)