Synthesis of structurally controlled noble metal nano- and micro-materials and their applications for colorimetric detection of biomolecules

다국어 초록 (Multilingual Abstract)

Nobel metal nanomaterials (NMNs) have been of significant interest owing to their shape-dependent electrical, optical, magnetic, and chemical properties. Particularly, NMNs comprised of gold or silver (or both) exhibit distinctive shape-dependent physical and chemical properties, which leads to myriad of research in NMNs with high anisotropy. One of the most frequently utilized method for synthesizing anisotropic NMNs is seed-mediated synthesis. In detail, highly monodispersed anisotropic NMNs can be obtained by introducing preformed nanoparticle seeds into a growth solution containing shape-directing agents, weak reductant, and metal precursor. This method also offers an ideal model system for the elucidation of mechanisms involved in the atomistic deposition and assembly process. We have been thoroughly investigated the growth of noble metal nanoparticle seeds and the characteristics of the resultant anisotropic nanostructures. Additionally, we also have investigated synthesis of gold microstructures in deep euteic solvent which is a kind of nascent green alternative for ionic liquid. Furthermore, the obtained NMNs are employed in colorimetric detection for various targets that are highly related with biological system including cancer gene, DNA binders and heavy metal ions after surface functionalization with DNA.

목차 (Table of Contents)

• Abstract --------------------------------------------------------------------- i
• Contents --------------------------------------------------------------------- iii
• List of Scheme ------------------------------------------------------------- vi
• List of Figure --------------------------------------------------------------- vii
• Chapter 1. Introduction ------------------------------------------------- 1
• 1.1 Noble Metal Nanomaterials ---------------------------- 2
• 1.2 Seed-Mediated Synthesis -------------------------------- 3
• 1.3 Surface Functionalization -------------------------------- 5
• 1.4 Thesis Structure ------------------------------------------- 7
• Chapter 2. Synthesis of Noble Metal Nano- and Micro-Materials 8
• 2.1 Combinatorial Polymer Library Approach to
• Control Seed Defects for the Synthesis of Silver
• Nanoplates
• 2.1.1 Introduction ------------------------------------------ 9
• 2.1.2 Experimental ---------------------------------------- 11
• 2.1.3 Result and discussion ------------------------------ 13
• 2.1.4 Conclusion ------------------------------------------- 34
• 2.2 H2O2-Assisted One-pot Synthesis of Silver
• Nanoplates Using Polymeric Materials
• 2.2.1 Introduction ----------------------------------------- 35
• 2.2.2 Result and discussion ------------------------------ 36
• 2.2.3 Conclusion -------------------------------------------- 41
• 2.3 Synthesis of Large Bumpy Silver Nanostructures
• with Controlled Sizes and Shapes for Catalytic
• Applications
• 2.3.1 Introduction ------------------------------------------ 42
• 2.3.2 Experimental ---------------------------------------- 44
• 2.3.3 Result and discussion ------------------------------- 46
• 2.3.4 Conclusion -------------------------------------------- 58
• 2.4 'In-plate' and 'On-plate' Structural Control of
• Ultrahigh Stable Gold/Silver Bimetallic Nanoplates as
• Redox Catalysts, Nanobuilding Blocks, and Single-
• Nanoparticle Surface-Enhanced Raman Scattering
• Probes
• 2.4.1 Introduction ------------------------------------------ 59
• 2.4.2 Experimental ---------------------------------------- 61
• 2.4.3 Result and discussion ------------------------------ 65
• 2.4.4 Conclusion ------------------------------------------- 88
• 2.5 Assembling Gold Nanocubes into a Nanoporous
• Gold Material
• 2.5.1 Introduction ----------------------------------------- 89
• 2.5.2 Result and discussion ------------------------------- 92
• 2.5.3 Conclusion ------------------------------------------- 100
• 2.6 Synthesis of Gold Microstructures with Surface
• Nanoroughness Using a Deep Eutectic Solvent for
• Catalytic and Diagnostic Applications
• 2.6.1 Introduction ----------------------------------------- 101
• 2.6.2 Experimental ---------------------------------------- 103
• 2.6.3 Result and discussion ------------------------------- 105
• 2.6.4 Conclusion -------------------------------------------- 115
• Chapter 3. Colorimetric Sensing Applications --------------------- 116
• 3.1 Salt Concentration-Induced Dehybridisation of
• DNA-Gold Nanoparticle Conjugate Assemblies for
• Diagnostic Applications
• 3.1.1 Introduction ----------------------------------------- 117
• 3.1.2 Experimental ----------------------------------------- 120
• 3.1.3 Result and discussion ------------------------------- 124
• 3.1.4 Conclusion ------------------------------------------- 132
• 3.2 Designed Hybridization Properties of DNA-Gold
• Nanoparticle Conjugates for the Ultraselective
• Detection of a Single-Base Mutation in the Breast
• Cancer Gene BRCA1
• 3.2.1 Introduction ------------------------------------------- 133
• 3.2.2 Experimental ----------------------------------------- 135
• 3.2.3 Result and discussion ------------------------------- 139
• 3.2.4 Conclusion -------------------------------------------- 152
• 3.3 Room-Temperature Colorimetric Detection of
• Coralyne Using DNA-Functionalized Nanoparticle
• Probes
• 3.3.1 Introduction ------------------------------------------ 153
• 3.3.2 Experimental ---------------------------------------- 155
• 3.3.3 Result and discussion ------------------------------ 157
• 3.3.4 Conclusion ------------------------------------------- 163
• 3.4 Colorimetric Detection of Acetylcholine with
• Plasmonic Nanomaterials Signaling
• 3.4.1 Introduction ----------------------------------------- 164
• 3.4.2 Experimental ---------------------------------------- 166
• 3.4.3 Result and discussion ----------------------------- 168
• 3.4.4 Conclusion ---------------------------------------- 186
• Chapter 4. Conclusion ------------------------------------------------- 187
• References --------------------------------------------------------------- 189