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      굴 Crassostrea gigas 부착기 유생의 부착에 미치는 신경전달물질종의 영향 = The Effect of Neuroactive Compounds on Settlement of Pacific Oyster, Crassostrea gigas Pediveliger Larvae

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      https://www.riss.kr/link?id=A105087472

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      국문 초록 (Abstract)

      굴 부착기 유생의 부착 유인 효과를 조사하기 위하여 6종류 (L-3,4-dihydroxyphenylalanine, serotonin, ${\gamma}$-amino butyric acid, norepinephrine, epinephrine, methyl bromide)의 신경전달물질을 채묘기질에 직접 처리하여 굴 부착기 유생의 부착 유인 효과를 조사하였다. 조사결과 평균 채묘율는 각각 $29.1{\pm}2.2%$, $35.4{\pm}2.0%$, $19.2{\pm}2.1%$, $11.0{\pm}1.2%$, $15.2{\pm}0.9%$ 및 $42.7{\pm}2.7%$ 로 MB 처리구에서 가장 높은 결과를 보였고, norepinephrine 처리구에서 유의적으로 가장 낮은 결과를 보였다 (P < 0.05). 채묘기질 $cm^2$당 부착밀도와 부착빈도는 ${\gamma}$-amino butyric acid와 methyl bromide 처리구에서 각각 $1.97{\pm}1.42$ 및 $2.37{\pm}1.86$ 마리/$cm^2$로 가장 높은 부착밀도를 보였다 (P < 0.05). 본 실험결과 굴 인공종묘배양장 등에서 부착기 유생의 부착요율을 높이는데, ${\gamma}$-amino butyric acid와 Methyl bromide를 이용하면 효과적일 것으로 보인다.
      번역하기

      굴 부착기 유생의 부착 유인 효과를 조사하기 위하여 6종류 (L-3,4-dihydroxyphenylalanine, serotonin, ${\gamma}$-amino butyric acid, norepinephrine, epinephrine, methyl bromide)의 신경전달물질을 채묘기질에 직접 처리...

      굴 부착기 유생의 부착 유인 효과를 조사하기 위하여 6종류 (L-3,4-dihydroxyphenylalanine, serotonin, ${\gamma}$-amino butyric acid, norepinephrine, epinephrine, methyl bromide)의 신경전달물질을 채묘기질에 직접 처리하여 굴 부착기 유생의 부착 유인 효과를 조사하였다. 조사결과 평균 채묘율는 각각 $29.1{\pm}2.2%$, $35.4{\pm}2.0%$, $19.2{\pm}2.1%$, $11.0{\pm}1.2%$, $15.2{\pm}0.9%$ 및 $42.7{\pm}2.7%$ 로 MB 처리구에서 가장 높은 결과를 보였고, norepinephrine 처리구에서 유의적으로 가장 낮은 결과를 보였다 (P < 0.05). 채묘기질 $cm^2$당 부착밀도와 부착빈도는 ${\gamma}$-amino butyric acid와 methyl bromide 처리구에서 각각 $1.97{\pm}1.42$ 및 $2.37{\pm}1.86$ 마리/$cm^2$로 가장 높은 부착밀도를 보였다 (P < 0.05). 본 실험결과 굴 인공종묘배양장 등에서 부착기 유생의 부착요율을 높이는데, ${\gamma}$-amino butyric acid와 Methyl bromide를 이용하면 효과적일 것으로 보인다.

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      참고문헌 (Reference)

      1 허영백, "참굴 Crassostrea gigas 인공종묘생산 시 유생의 성장과 체성분 변화" 한국양식학회 21 (21): 203-212, 2008

      2 전창영, "굴 Crassostrea gigas 부착기 유생의 부착에 미치는 수온 및 염분의 영향" 한국패류학회 28 (28): 21-28, 2012

      3 Morse, D. E., "γ-Aminobutyric acid, a neurotransmitter, induces planktonic abalone larvae to settle and begin metamorphosis" 204 : 407-410, 1979

      4 Okano, K., "Visualization of cement exocytosis in the cypris cement gland of the barnacle Megabalanus rosa" 199 : 2131-2137, 1996

      5 Walch, M., "Use of l-DOPA and soluble bacterial products to improve set of Crassostrea virginica (Gmelin, 1791) and C. gigas (Thunberg, 1793)" 18 : 133-138, 1999

      6 Ganesan. A. M., "The role of bacterial biofilms and exudates on the settlement of mussel (Perna canaliculus) larvae" 306 : 388-392, 2010

      7 Leitz, T., "The marine bacterium Alteromonas espejiana induces metamorphosis of the hydroid Hydractinia echinate" 115 : 173-178, 1993

      8 Faimali, M., "The interplay of substrate nature and biofilm formation in regulating Balanus amphitrite Darwin, 1854 larval settlement" 306 : 37-50, 2004

      9 Yu, X., "The effect of chemical cues on settlement of pearl oyster Pinctada fucata martensii (Dunker) larvae" 277 : 83-91, 2008

      10 Doroudi, M.S., "The effect of chemical cues on settlement behaviour of blacklip pearl oyster (Pinctada margaritifera) larvae" 209 : 117-124, 2002

      1 허영백, "참굴 Crassostrea gigas 인공종묘생산 시 유생의 성장과 체성분 변화" 한국양식학회 21 (21): 203-212, 2008

      2 전창영, "굴 Crassostrea gigas 부착기 유생의 부착에 미치는 수온 및 염분의 영향" 한국패류학회 28 (28): 21-28, 2012

      3 Morse, D. E., "γ-Aminobutyric acid, a neurotransmitter, induces planktonic abalone larvae to settle and begin metamorphosis" 204 : 407-410, 1979

      4 Okano, K., "Visualization of cement exocytosis in the cypris cement gland of the barnacle Megabalanus rosa" 199 : 2131-2137, 1996

      5 Walch, M., "Use of l-DOPA and soluble bacterial products to improve set of Crassostrea virginica (Gmelin, 1791) and C. gigas (Thunberg, 1793)" 18 : 133-138, 1999

      6 Ganesan. A. M., "The role of bacterial biofilms and exudates on the settlement of mussel (Perna canaliculus) larvae" 306 : 388-392, 2010

      7 Leitz, T., "The marine bacterium Alteromonas espejiana induces metamorphosis of the hydroid Hydractinia echinate" 115 : 173-178, 1993

      8 Faimali, M., "The interplay of substrate nature and biofilm formation in regulating Balanus amphitrite Darwin, 1854 larval settlement" 306 : 37-50, 2004

      9 Yu, X., "The effect of chemical cues on settlement of pearl oyster Pinctada fucata martensii (Dunker) larvae" 277 : 83-91, 2008

      10 Doroudi, M.S., "The effect of chemical cues on settlement behaviour of blacklip pearl oyster (Pinctada margaritifera) larvae" 209 : 117-124, 2002

      11 Min, K. S., "Studies on rearing conditions for mass seedling production in Pacific oyster larvae, Crassostrea gigas (Thunberg)" 49 : 91-111, 1995

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      19 Alfaro, A.C., "Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms" 322-323 : 158-168, 2011

      20 Morse, D. E., "Recent progress in larval settlement and metamorphosis: closing the gaps between molecular biology and ecology" 46 : 465-483, 1990

      21 Greene, J.K., "Oyster (Crassostrea virginica Gmelin) restoration studies in the Great Bay estuary" NOAA 2005

      22 Crisp, D. J., "Overview of research on marine invertebrate larvae, In Chemoreception in Marine Organisms, vol. 1" Academic Press 177-265, 1984

      23 Osborne, N. N., "Occurrence of GABA and taurine in the nervous systems of the dogfish and some invertebrates" 2 : 433-438, 1971

      24 Tamburri, M. N., "Natural sources and properties of chemical inducers mediating settlement of oyster larvae: a re-examination" 183 : 327-338, 1992

      25 Hadfield, M.G., "Natural chemical cues for settlement and metamorphosis of marine-invertebrate larvae, In Marine Chemical Ecology" CRC press 431-461, 2001

      26 Pawlik, J. R., "Natural and artificial induction of metamorphosis of Phragmatopoma lapidosa californica (Polychaeta: Sabellariidae), with a critical look at the effects of bioactive compounds on marine invertebrate larvae" 46 : 512-536, 1990

      27 Hadfield, M. G., "Metamorphosis in marine molluscan larvae: an analysis of stimulus and response, In Settlement and Metamorphosis of Marine Invertebrate Larvae" Elsevier 165-175, 1978

      28 Zhao, B., "Larval settlement of the silver- or goldlip pearl oyster Pinctada maxima (Jameson) in response to natural biofilms and chemical cues" 220 : 883-901, 2003

      29 Qian, P. Y., "Larval settlement of polychaetes" 402 : 239-253, 1999

      30 Murthy, P. S., "Larval settlement and surfaces: implications in development of antifouling strategies, In Marine and Industrial Biofouling" Springer 233-263, 2009

      31 Zhao, B., "Larval settlement and metamorphosis in the slipper limpet Crepidula onyx (Sowerby) in response to conspecific cues and the cues from biofilm" 269 : 39-51, 2002

      32 Yang, J. L., "Larval metamorphosis of the mussel Mytilus galloprovincialis Lamarck, 1819 in response to neurotransmitter blockers and tetraethylammonium" 27 : 193-199, 2011

      33 Andrews, W. R., "Isolation and characterization of the metamorphic inducer of the common mud crab, Panopeus herbstii" 261 : 121-134, 2001

      34 Baloun, A.J., "Ionic control of settlement and metamorphosis in larval Haliotis rufescens (Gastropoda)" 167 : 124-138, 1984

      35 Sánchez-Lazo, C., "Induction of settlement in larvae of the mussel Mytilus galloprovincialis using neuroactive compounds" 344-349 : 210-215, 2012

      36 Martinez, G., "Induction of settlement and metamorphosis of the scallop Argopecten purpuratus Lamarck by excess K+ and epinephrine: energetic costs" 18 : 41-46, 1999

      37 Coon, S. L., "Induction of settlement and metamorphosis of the Pacific oyster, Crassostrea gigas (Thunberg), by L-DOPA and catecholamines" 94 : 211-221, 1985

      38 Yang, J. L., "Induction of metamorphosis of pediveliger larvae of the mussel Mytilus galloprovincialis Lamarck, 1819 using neuroactive compounds, KCl, NH4Cl and organic solvents" 24 : 461-470, 2008

      39 Beiras, R., "Induction of metamorphosis in larvae of the oyster Crassostrea gigas using neuroactive compounds" 123 : 327-334, 1995

      40 Fang, Q., "Induction of larval settlement and metamorphosis of two oysters Crassostrea gigas and Ostrea cucullata by some chemicals" 20 (20): 20-26, 2001

      41 Kang, K. H., "Induction of larval settlement and metamorphosis of the abalone, Haliotis discus hannai larvae using bromomethane and potassium chloride" 230 : 249-259, 2004

      42 Gao, R. C., "Induction of larval settlement and metamorphosis of Coelomactra antiquata using some chemicals" 30 (30): 597-602, 2006

      43 Henderson, B. A., "Handling and Remote Setting Techniques for the Pacific oyster larvae, Crassostrea gigas" Oregon State University 1983

      44 Min. S. K., "Growth and survival of the artificial and natural seeding in the Pacific oyster, Crassostrea gigas (Thunberg)" 57 : 43-53, 1999

      45 Maki, J. S., "Factors controlling attachment of bryozoan larvae: a comparison of bacterial films and unfilmed surfaces" 177 : 295-302, 1989

      46 Teh, C. P., "Epinephrine and l-DOPA promote larval settlement and metamorphosis of the tropical oyster, Crassostrea iredalei (Faustino, 1932): An oyster hatchery perspective" 338 (338): 260-263, 2012

      47 Li, H. F., "Enhancement of larval settlement and metamorphosis through biological and chemical cues in the abalone Haliotis diversicolor suertexta" 258 (258): 416-423, 2006

      48 Barlow, L. A., "Electrophysiological and behavioral responses of larvae of the red abalone (Haliotis rufescens) to settlement-inducing substances" 46 : 537-554, 1990

      49 Wassnig, M., "Effects of settlement cues on behaviour and substrate attachment of hatchery reared winged pearl oyster (Pteria penguin) larvae" 344-349 : 216-222, 2012

      50 Yang, J. L., "Effects of neuroactive compounds, ions and organic solvents on larval metamorphosis of the mussel Mytilus coruscus" 396-399 : 106-112, 2013

      51 Mesías-Gansbiller, C., "Effects of chemical cues on larval settlement of the flat oyster (Ostrea edulis L.): A hatchery approach" 376-379 : 85-89, 2013

      52 García-Lavandeira, M., "Effects of GABA and epinephrine on the settlement and metamorphosis of the larvae of four species of bivalve molluscs" 316 : 149-156, 2005

      53 Weiner, R. M., "Effect of biofilms of the marine bacterium Alteromonas colwelliana (LST) on set of the oysters Crassostrea gigas (Thunberg, 1793) and C. virginica (Gmelin, 1791)" 8 : 117-123, 1989

      54 Taniguchi, K., "Dibromomethane, a chemical inducer of larval settlement and metamorphosis of the sea urchin, Strongylocentrotus nudus" 60 : 795-796, 1994

      55 Bonar, D.B., "Control of oyster settlement and metamorphosis by endogenous and exogenous chemical cues" 46 : 484-498, 1990

      56 Coon, S.L., "Competence and delay of metamorphosis in the Pacific oyster Crassostrea gigas" 106 (106): 379-387, 1990

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      58 Grant, M. N., "Chemical induction of settlement behavior in larvae of the eastern oyster Crassostrea virginica (Gmelin)" 402-403 : 84-91, 2013

      59 Zimmer-Faust, R.K., "Chemical identity and ecological implications of a waterborne, larval settlement cue" 39 : 1075-1087, 1994

      60 Pawlik, J. R., "Chemical ecology of the settlement of benthic marine invertebrate. Oceanogr" 30 : 273-335, 1992

      61 Johnson, C. R., "Characteristic bacteria associated with surfaces of coralline algae: a hypothesis for bacterial induction of marine invertebrate larvae" 74 : 281-294, 1991

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      64 Coon, S. L., "Ammonia induces settlement behavior in oyster larvae" 179 (179): 297-303, 1990

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