Stability of citrate-capped silver nanoparticles in exposure media and their effects on the development of embryonic zebrafish (Danio rerio)

다국어 초록 (Multilingual Abstract)

The stability of citrate-capped silver nanoparticles(AgNPs) and the embryonic developmental toxicitywere evaluated in the fish test water. Serious aggregation ofAgNPs was observed in undiluted fish water (DM-100) inwhich high concentration of ionic salts exist. However,AgNPs were found to be stable for 7 days in DM-10,prepared by diluting the original fish water (DM-100) withdeionized water to 10 %. The normal physiology of zebrafishembryos were evaluated in DM-10 to see if DM-10can be used as a control vehicle for the embryonic fishtoxicity test. As results, DM-10 without AgNPs did notinduce any significant adverse effects on embryonicdevelopment of zebrafish determined by mortality, hatching,malformations and heart rate. When embryonic toxicityof AgNPs was tested in both DM-10 and in DM-100,AgNPs showed higher toxicity in DM-10 than in DM-100.
This means that the big-sized aggregates of AgNPs werelow toxic compared to the nano-sized AgNPs. AgNPsinduced delayed hatching, decreased heart rate, pericardialedema, and embryo death. Accumulation of AgNPs in theembryo bodies was also observed. Based on this study,citrate-capped AgNPs are not aggregated in DM-10 and itcan be used as a control vehicle in the toxicity test of fishembryonic development.

참고문헌 (Reference)

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