Modulation of Gene Expression in Raw 264.7 Macrophage Cells by Saccharomyces exiguus and LPS|RISS 상세보기

다국어 초록 (Multilingual Abstract)

Macrophages can recognize antigens and microorganisms, then initiate an appropriate defense. However, there has been a lack of comprehensive information regarding the genes which is modulated by commensal yeasts including Saccharomyces cerevisiae or Saccharomyces exiguus. Moreover, it is not clear to what extent the beneficial yeasts modulate the immune response against microbes and/or microbial toxins. We studied interactions between host cells and yeast/bacterial toxin (LPS) by analyzing the transcriptional response of macrophages stimulated by the Saccharomyces exiguus and/or Lipopolysaccharides with DNA microarray, which contains about 25,000 genes. Thirty three genes were identified to be modulated by more than 2 folds between groups of macrophage cells. Pathway analysis provided insight into the mutual interactions. Of particular interest was the responses elicited by the fungus in the murine macrophage cells, including modulation of immunity/defense, cellular signal transduction, cell proliferation/differentiation and transport. This indicates that the yeast induces immune response pathways as well as those associated with cell proliferation and transport. Among the 33 genes identified from the DNA microarray screening, 8 genes were further checked by RT-PCR analysis using gene specific primers. Compared to those of negative control, the sequential treatment with the yeast strain then LPS apparently induced the expression of Tnfaip3, IL7R and CD86, while it inhibited the expression of Cxcl10 and CD83. In conclusion, this study identified the genes that are up-regulated by Saccharomyces exiguus and it remains to be further studied whether these genes are modulated at the protein level, and also for their roles in controlling immune responses.

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참고문헌 (Reference)

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