지하수 세균 군집의 유전적 다양성 = The Genetic Diversity of Bacterial Communities in the Groundwater

다국어 초록 (Multilingual Abstract)

In order to characterize the genetic diversity of bacterial community in groundwater, samples were collected from used for drinking water and polluted with heavy metal wastewater in Seoul city and natural cave of Kangwondo. The DNA was amplified with 16S rDNA-based primers by use of the PCR, and then analysed ARDRA (amplified ribosomal DNA restriction analysis). Restriction endonuclease analysis patterns of amplified 16S rDNA in drinking water and wastewater relatively showed high genetic diversity in situ and drinking groundwater. The number of DNA fragments varied with in situ and drinking water. This method of ARDRA of bacterial communities in groundwater could be used for a quick assessment of genotypic changes between different locations reflecting different environmental conditions and the diversity reflected pollution of groundwater(natural cave water>drinking water>waste water, as in order of grade). [Genetic diversity, Groundwater, 16S rDNA, PCR, ARDRA].