바이러스성 출혈성 패혈증에 감염된 넙치의 cDNA microarray 분석 : 수온에 따른 면역 유전자 발현의 차이 = cDNA microarray analysis of viral hemorrhagic septicemia infected olive flounder, Paralichthys olivaceus: immune gene expression at different water temperature

다국어 초록 (Multilingual Abstract)

The olive flounder, Paralichthys olivaceus is susceptible to viral hemorrhagic septicaemia virus (VHSV) at 15℃ but no mortality at 20℃ even though the virus can grow well in vitro at 20℃. Thus, we designed an experiment to know immune response of olive flounder against VHSV when the host reared at 15℃ or 20℃. cDNA microarray analysis was performed to compare the gene expression patterns of the kidney cells between the host reared at 15℃ or 20℃. The expression of MHC class I, IL-8, myeloperoxidae and endonuclease G-like having function for the antigen presentation and chemokine-factor were up-regulted both the 15℃ and 20℃ during VHSV infection. MHC class Ⅱ gene existing on antigen-presenting cells and B cell lymphocytes, immunoglobulin (Ig) genes and phagocytosis related genes were down-regulated at 15℃ but highly expressed at 20℃. It can be thought that innate immune related antigen presentation by MHC class Ⅰ and phagocytosis reaction against VHSV are efficiently occur both the temperature but macrophage or B cell related antigen presentation via MHC class II fails to induce downstream immune reactions (adaptive immunity) to make antibody, and it can be one of the reason that causes high mortality only at 15℃.