Mucosubstance Histochemistry of the Epidermis in Yellowtail, Striped Beakperch, Brown Spotted Grouper, Sea Chub and Multicolorfin Rainbowfish

다국어 초록 (Multilingual Abstract)

To investigate the properties of mucosubstancesof the epidermis in various teleostean species, conventionalhistochemical stainings were used on the skin in five speciesof order Perciformes, i. e., yelowtail, Seriola quinqueradiat,striped beakperch, Oplegnathus fasciatus, brown spottedgrouper, Epinephelus chlorostigma, sea chub, Ditrematemincki Halichoerespoecilopterus. The following methods were used: periodicacid Schif (PAS), alcian blue (AB) pH at 2.5, AB pH at 1.0,AB pH at 2.5-PAS, AB pH at 1.0-PAS, aldehyde fuchsin (AF)pH at 1.7-AB pH at 2.5 and high iron diamine (HID)-AB pHat 2.5. The epidermis of all five species consisted of threelayers: superficial, middle, and basal layer. The superficiallayer was comprised of rather flatened cells. In particular,the outermost layer of striped beakperch and middle layer ofsea chub consisted of mucus-secreting cells. Mucous cells,number in diferent body regions and species. Althoughthere was a slight diference in the amount in variousspecies and body regions, the secretory contents of themucous cells in the five teleostean species contained acidicmucopolysaccharides. In yellowtail, striped beakperch, andmulticolorfin rainbowfish, the property of mucosubstanceswas identified as sialomucin, while it was sulphomucin inbrown spotted grouper and sea chub.

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