마우스 귀부종 시험법과 국소임파절시험법을 병합한 피부감작시험법 개발에 대한 연구 = Development of a skin sensitization method through consolidation of mouse ear swelling test and mouse Local Lymph node assay

다국어 초록 (Multilingual Abstract)

In the present both mouse ear swelling test and mouse local lymph node assay are considered reliable method for determination of chemicals` type IV contact hypersensitivity capability. We developed a skin sensitization method through consolidation of mouse ear swelling test and mouse local lymph node assay. Sensitization of BALB / c mice with potassium dichromate, a well known skin sensitizer, was established through intra－dermal injection of emulsion containing 50 ㎕ of 20 mM potassium dichromate and 50 ㎕ complete Fleund`s adjuvant (CFA) into both flanks. Control mice were treated with CFA only. Thereafter, 10 days later, mice were challenged by injecting 20 ㎕of 3.5 mM potassium dichromate into pinnae of one ear and saline vehicle into that of another ear. Increment of ear thickness was determined using ear thickness guage 48 hour later of the challenge. In vitro BrdU proliferation assay was undertaken using auricular lymph node cells in the presence (1, 10 nM) or absence of potassium dichromate. Number of lymph node cells was significantly elevated in the chrome－sensitized / challenged mice than the other control mice. Ear swelling index, obtained through subtracting ear thickness before challenge from that after the challenge, was also significantly higher in the chrome－sensitized / challenged mice. Furthermore, in vitro auricular lymph node cell proliferation index was nearly two－fold higher at the chrome－sensitized / challenged mice than the other control mice. In order to be evaluated as an alternative skin sensitization test, our method deserves more repetition using various chemical sensitizers and irritants