종합비타민의 랫드에서 반복투여독성 시험과 대식세포 기능 활성 평가 = Evaluation of Macrophage Activity and Repeated Oral Dose Toxicity in Sprague-Dawley Rats on Multivitamin

다국어 초록 (Multilingual Abstract)

The objective of this study is to investigate the effect of multivitamin on macrophage activity in Raw 264.7 cell and repeated oral dose toxicity in Sprague-Dawely rat of multivitamin. Raw 264.7 cells were treated with 50 and 100 μg/mL multivitamin for 24 h. To measure the activity of macrophages, NO and TNF-α assays were performed in Raw 264.7 cells. Treatment with 50 and 100 μg/mL multivitamin for 24 h significantly increased production of NO and TNF-α compared with control groups, indicating activation of macrophages. The female rats were treated with multivitamin of control group, low group (0.24 g/kg), medium group (1 g/kg) and high group (2 g/kg)intragastrically for 4 weeks, respectively. We examined the body weight, the feed intake, the clinical signs and serum biochemical analysis. We also observed the histopathological changes of liver, ovary, brain, adrenal gland, spleen, kidney,heart and lung in rats. No significant differences in body weights, feed intake, biochemical analysis and histopathological observations between control and multivitamin treatment group were found. In conclusion, multivitamin is physiologically safe and improve macrophage activity.

국문 초록 (Abstract)

본 연구는 Spragye-Dawely 계통의 암컷 랫드에서 종합비타민의 반복경구투여 독성평가와 대식세포 Raw 264.7세포의 NO 및 TNF-α assay를 통한 면역 활성을 평가하기 위해서 실시하였다. 종합비타민을 대식세포의 활성능을 측정하기 위해 Raw 264.7 세포에서 NO와 TNF-α의생성을 측정하였다. 종합비타민을 대식세포에 24시간 처리한 결과 대조군과 비교 시 NO와 TNF-α가 유의적으로상승하였다. 이 결과 종합비타민이 대식세포인 Raw 264.7세포를 활성화시키는 것으로 사료된다. 또한 랫드에서 종합비타민의 독성평가를 위하여 랫드에 종합비타민을 0.24 g/kg, 1 g/kg 그리고 2 g/kg을 4주 동안 경구투여를 하였다.
종합비타민의 안전성을 확인하기 위해 다음과 같은 관찰및 검사를 하였다. 검사항목으로는 체중과 사료 섭취량,임상증상, 혈청생화학적 검사를 관찰한 결과 대조군과 투여군을 비교 시 유의적인 변화가 나타나지 않았다. 따라서 종합비타민은 생리대사에 무해하며 면역증강의 효과를나타내는 것으로 사료된다.

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