훈증소독제, Fumagari OPP®의 Staphylococcus aureus에 대한 살균효과 = Bactericidal Efficacy of Fumagari OPP®,Fumigant Against Staphylococcus aureus

다국어 초록 (Multilingual Abstract)

This study was performed to evaluate the bactericidal efficacy of Fumagari OPP®, fumigation disinfectant,containing 20% ortho-phenylphenol against Staphylococcus aureus (S. aureus). In this research, efficacy test of fumigant against S. aureus was carried out according to French standard NF T 72-281. S. aureus working culture suspension number (N value), all of the colony numbers on the carriers exposed with the fumigant (n1, n2, and n3), the number of bacterial test suspentions by pour plate method (N1), the number of bacterial test suspentions by filter membrane method (N2) and the mean number of bacteria recovered on the control-carriers (T value) were obtained from the preliminary test. In addition, the reduction number of S. aureus exposed with the fumigant (d value)was calculated using T value, the mean number of bacteria in recovery solution (n'1) and the mean number of bacteria on carriers plated in agar (n'2). N value was 4.0 × 108CFU/mL, and n1, n2, and n3 were higher than 0.5N1, 0.5N2 and 0.5N1, respectively. Additionally, T value was 3.4 × 106 CFU/mL. In the bactericidal effect of the fumigant, the d value was 6.43 logCFU/mL. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. The results indicated that Fumagari OPP® had an effective bactericidal activity against S. aureus, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with pathogenic bacteria.

국문 초록 (Abstract)

본 연구는 20% ortho-phenylphenol을 함유한 훈증소독제, Fumagari OPP®의 Staphylococcus aureus (S. aureus)에대한 살균효과를 평가하기 위해, French standard NF T72-281에 따라 수행하였다. 배양 현탁액 중 S. aureus의 균수 (N 값), 훈증소독제에 노출된 각 담체의 균수 (n1, n2,n3), 평판배지법에 의한 시험균주 현탁액 중 균수 (N1), 여과법에 의한 시험균주 현탁액 중 균수 (N2), 그리고 대조담체의 회복 균수의 평균값 (T 값)을 예비실험을 통해 구하였다.
또한, 훈증소독제에 노출된 S. aureus의 감소 균수 (d 값)는, T 값, 회복액 중 균수의 평균값 (n'1) 그리고 배지의담체에서 증식한 균수의 평균값 (n'2) 등을 이용하여 산출하였다. N 값은 4.0 × 108 CFU/mL이었으며, n1, n2, n3은각각 0.5N1, 0.5N2, 0.5N1 보다 높게 나타났다. 그리고 T값은 3.4 × 106CFU/mL이었다. 훈증소독제의 살균효과에있어서, d 값은 6.43 logCFU/mL이었다. 훈증소독제에 대한 프랑스 기준에 따르면, 효과적인 살균력을 갖는 훈증소독제의 d 값이 5 logCFU/mL 이상이어야 하는 것으로규정하고 있다. 본 연구의 결과로부터, Fumagari OPP®는S. aureus에 대해 높은 살균효과를 갖는 것으로 나타나, 병원성 세균으로 오염된 식품재료와 주방기기의 소독에 적용할 수 있을 것으로 사료된다.

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