Antihyperglycemic Effect of Crude Extracts of Some Egyptian Plants and Algae

다국어 초록 (Multilingual Abstract)

Diabetes mellitus is a major global health problem. Various plant extracts have proven antidiabetic activity and are considered as promising substitution for antidiabetic drugs. The antihyperglycemic effect of 16 plants and 4 algae, commonly used in Egypt for the treatment of diabetes mellitus, was investigated. A diabetes model was induced by intraperitoneal injection of nicotinamide (120 mg/kg body weight [b.wt.]), then streptozotocin (200 mg/kg b.wt.) after 15 min. Hydroethanolic extracts (80%) of the plants and algae under investigation were prepared. The extracts were orally administered to nicotinamide-streptozotocin–induced diabetic mice by a gastric tube at doses 10 or 50 mg/kg b.wt. for 1 week. The antidiabetic activity was assessed by detection of serum glucose concentrations at the fasting state and after 2 h of oral glucose loading (4.2 mg/kg b.wt.). Extracts prepared from Cassia acutifolia, Fraxinus ornus, Salix aegyptiaca, Cichorium intybus, and Eucalyptus globulus showed the highest antihyperglycemic activity among the tested plants. Extracts prepared from Sonchus oleraceus, Bougainvillea spectabilis (leaves), Plantago psyllium (seeds), Morus nigra (leaves), and Serena repens (fruits) were found to have antihyperglycemic potentials. Extracts prepared from Caulerpa lentillifera and Spirulina versicolor showed the most potent antihyperglycemic activity among the tested algae. However, some of the tested plants have insulinotropic effects, all assessed algae have not. Identification of lead compounds from these plants and algae for novel antidiabetic drug development is recommended.

참고문헌 (Reference)

1 Donnelly R, "Vascular complications of diabetes" 320 : 1062-1066, 2000

2 Al-adhal A, "The effect of Cassia angustifolia (senna) leaves on the fasting blood sugar in a sample of mild diabetic Yemeni patients" 1 : 1-6, 2009

3 AbouZid SF, "Survey on medicinal plants and spices used in Beni-Sueif, Upper Egypt" 7 : 1-6, 2011

4 Kapoor LD, "Survey of Indian plants for saponins, alkaloids and flavonoids" 32 : 297-304, 1969

5 Eddouks M, "Study of the hypoglycaemic activity of Lepidium sativum L. aqueous extract in normal and diabetic rats" 97 : 391-395, 2005

6 Afifi NA, "Some pharmacological activities of essential oils of certain umbelliferous fruits" 42 : 85-92, 1994

7 World Health Organization, "Quality Control Methods for Medicinal Plant Materials" 1998

8 Roa M, "PC-STAT"

9 Bedekar A, "Natural products for type II diabetes treatment" 71 : 21-72, 2010

10 황완균, "Natural compounds, fraxin and chemicals structurally related to fraxin protect cells fromoxidative stress" 생화학분자생물학회 37 (37): 436-446, 2005

11 Andrade-Cetto A, "Mexican plants with hypoglycaemic effect used in the treatment of diabetes" 99 : 325-348, 2005

12 Rodriguez-Moran M, "Lazcano-Burciaga G : Lipid-and glucose-lowering efficacy of Plantago psyllium in type II diabetes" 12 : 273-278, 1998

13 Ahmed MA, "Induction of biologically active flavonoids in cell cultures of Morus nigra and testing their hypoglycemic efficacy" 79 : 951-961, 2011

14 Kobayashi T, "Impairment of PI3-K/Akt pathway underlies attenuated endothelial function in aorta of type 2 diabetic mouse model" 44 : 956-962, 2010

15 Lawrence JC, "Human Pharmacology, Molecular to Clinical" Mosby Year Book 523-539, 1994

16 Shaw JE, "Global estimates of the prevalence of diabetes for 2010 and 2030" 87 : 4-14, 2010

17 Kostova L, "Fraxinus ornus L" 72 : 471-480, 2001

18 Swanston-Flatt SK, "Evaluation of traditionally plant treatments for diabetes, studies in streptozotocin diabetic mice" 26 : 51-55, 1989

19 Saeedi M, "Evaluation of binding properties of Plantago psyllium seed mucilage" 60 : 339-348, 2010

20 Paget LS, "Evaluation of Drug Activities" Academic Press 1964

21 Nakamura T, "Establishment and pathophysiological characterization of type 2 diabetic mouse model produced by streptozotocin and nicotinamide" 29 : 1167-1174, 2006

22 Shipra Gupta, "Elucidation of Mechanism of Action of Cassia auriculata Leaf Extract for Its Antidiabetic Activity in Streptozotocin-Induced Diabetic Rats" 한국식품영양과학회 13 (13): 528-534, 2010

23 Stefanova Z, "Effect of a total extract from Fraxinus ornus stem bark and esculin on zymosan-and carrageenan-induced paw oedema in mice" 46 : 101-106, 1995

24 Rizk AM, "Constituents of plants growing in Qatar. I. A chemical survey of sixty plants" 52 : 33-44, 1982

25 Patricia Matanjun, "Comparison of Cardiovascular Protective Effects of Tropical Seaweeds, Kappaphycus alvarezii, Caulerpa lentillifera, and Sargassum polycystum, on High-Cholesterol/High-Fat Diet in Rats" 한국식품영양과학회 13 (13): 792-800, 2010

26 Segelman AB, "Biological and phytochemical screening of plants. IV. A new rapid procedure for the simultaneous determination of saponins and tannins" 32 : 59-65, 1969

27 Ahmed OM, "Biochemical effects of Cichorium intybus and Sonchus oleraceus infusions and esculetin on streptozotocin-induced diabetic albino rats" 7 : 1124-1137, 2011

28 Marinova EM, "Antioxidative action of the ethanolic extract and some hydroxycoumarins of Fraxinus ornus bark" 51 : 125-132, 1994

29 Matanjun P, "Antioxidant activities and phenolics content of eight species of seaweeds from north Borneo" 20 : 367-373, 2008

30 Kostova IN, "Antimicrobial properties of some hydroxycoumarins and Fraxinus ornus bark extracts" 39 : 205-208, 1993

31 Ahmed OM, "Antihyperglycemic effects of water extract of Ulva lactuca and its polysaccharides in nicotinamide-streptozotocininduced diabetic rats" 54 : 273-297, 2010

32 Gray AM, "Antihyperglycemic actions of Eucalyptus globulus(Eucalyptus)are associated with pancreatic and extrapancreatic effects in mice" 128 : 2319-2323, 1998

33 Layam A, "Antidiabetic property of spirulina" 35 : 29-33, 2006

34 Maiti R, "Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocininduced diabetic rats" 92 : 85-91, 2004

35 Kumar S, "Antidiabetic and anti-lipemic effects of Cassia siamea leaves extract in streptozotocin induced diabetic rats" 3 : 871-873, 2010

36 Kaskoos RA, "Antidiabetic activity of Cichorium intybus L. seeds on STZ-induced diabetic rats" 3 : 161-164, 2012

37 Ahmed OM, "Anti-hyperlipidemic, antioxidant and cardiac improving effects of water extract of Ulva lactuca and its polysaccharides in nicotinamide-streptozotocin-induced diabetic rats" 54 : 253-272, 2010

38 Lee MS, "Anti-diabetic properties of chrysophanol and its glucoside from Rhubarb Rhizome" 31 : 2154-2157, 2008

39 Salahuddin M, "Anti-diabetic activity of aqueous bark extract of Cassia glauca in streptozotocin-induced diabetic rats" 88 : 153-160, 2010

40 Lee HJ, "Algae consumption and risk of type 2 diabetes. Korean National Health and Nutrition Examination Survey in 2005" 56 : 13-18, 2010

41 Prabhakar PK, "A target based therapeutic approach towards diabetes mellitus using medicinal plants" 4 : 291-308, 2008