호흡기 상피세포에서 MUC5AC와 MUC5B 발현에 대한 Anthocyanidin의 효과 = Effect of Anthocyanidin on MUC5AC and MUC5B Expression in Airway Epithelial Cells

다국어 초록 (Multilingual Abstract)

Background and Objectives Naringenin and delphinidin are types of anthocyanidin, which are flavonoids and thus have anti-inflammatory property. Moderate consumption of natural dietary naringenin and delphinidin is believed to do anti-inflammatory action, but the action mechanism is unclear. Therefore, this study aimed to investigate the effects of naringenin and delphinidin on interleukin-1β (IL-1β)- and lipopolysaccharide (LPS)-induced MUC5AC and MUC5B expressions in airway epithelial cells.
Materials and Method In NCI-H292 cells and cultured nasal polyp epithelial cells, the effects of naringenin and delphinidin on IL-1β- and LPS-induced MUC5AC and MUC5B expressions were analyzed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.
Results Delphinidin attenuated IL-1β- and LPS-induced MUC5AC and MUC5B mRNA and glycoprotein expression in a dose-dependent pattern in NCI-H292 cells and in cultured nasal polyp epithelial cells. Naringenin partially attenuated IL-1β- and LPS-induced MUC5AC and MUC5B mRNA and glycoprotein expression at a high dose.
Conclusion These results suggest that delphinidin attenuates MUC5AC and MUC5B expressions in the airway epithelial cells. Between anthocyanidin and delphinidin, delphinidin exhibits greater potential as an ideal therapeutic agent for the control of mucus-hypersecretion in the treatment of airway inflammatory diseases.

참고문헌 (Reference)

1 우현재, "호흡기 상피세포에서 roxithromycin에 의한 MUC5B와 MUC8 점액 유전자 발현 및 점액 생성의 억제" 대한이비인후과학회 51 (51): 617-622, 2008

2 Aviram M, "Wine flavonoids protect against LDL oxidation and atherosclerosis" 957 : 146-161, 2002

3 Ali MS, "Upper airway mucin gene expression: a review" 117 (117): 932-938, 2007

4 고은경, "TNF-α에 의해 유도된 세포부착분자의 발현에 대한 Delphinidin Chloride의 억제 효과" 한국환경생물학회 27 (27): 88-94, 2009

5 Shi Y, "Naringenin inhibits allergen-induced airway inflammation and airway responsiveness and inhibits NF-kappaB activity in a murine model of asthma" 87 (87): 729-735, 2009

6 Hertog MG, "Intake of potentially anticarcinogenic flavonoids and their determinants in adults in The Netherlands" 20 (20): 21-29, 1993

7 Fritz J, "Impact of delphinidin on the maintenance of DNA integrity in human colon carcinoma cells" 56 (56): 8891-8896, 2008

8 Lee JG, "Expression and regulation of MUC8 & MUC5AC by various cytokines in normal human nasal epithelial cells" 44 (44): 600-605, 2001

9 Boniface R, "Effect of anthocyanins on human connective tissue metabolism in the human" 209 (209): 368-372, 1996

10 Hertog MG, "Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study" 342 (342): 1007-1011, 1993

11 Yun JM, "Delphinidin, an anthocyanidin in pigmented fruits and vegetables, induces apoptosis and cell cycle arrest in human colon cancer HCT116 cells" 48 (48): 260-270, 2009

12 Chen CY, "Delphinidin attenuates stress injury induced by oxidized low-density lipoprotein in human umbilical vein endothelial cells" 183 (183): 105-112, 2010

13 Koponen JM, "Contents of anthocyanins and ellagitannins in selected foods consumed in Finland" 55 (55): 1612-1619, 2007

14 Wang H, "Antioxidant and antiinflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries" 62 (62): 294-296, 1999