菊花엽절편 培養에 있어서 植物體 再分化에 미치는 生長調節劑의 영향 = Efficiency of Growth Regulators on Regeneration in Leaf Segment Culture of Chrysanthemum

국문 초록 (Abstract)

菊花의生長點을 기내 無菌培養하여 얻은 어린 식물체의 上位 葉切片을 2,4-D와BA를 混合 添加한 MS배지에 치상하여 캘러스,植物體再分化,뿌리의 形成 등에 미치는 生長調節劑의 효과를 조사하였다.植物體分化및 뿌리발생은 2,4-D와 BA의 혼합처리농도에 따라 유의성 있는 차이가 인정되었으며 2,4-D×BA의交互作用 效果도 높게나타났다.
캘러스 및 分化植物體發生은 MS배지에 2,4-D0,5-2.0㎎/L에 BA 0.1~2.0㎎/L를 첨가한 배지에서 양호하였으며 특히 2,4-D 0.5㎎/L와 BA
0.5㎎/L를 혼합처리한 구에서 식물체분화율이 가장높았으며 切片體의가장자리에서 발생한 談錄色의 덩이에서 直接 植物體가 발생하였다.

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate effect of plant growth regulators on plant regeneration. Leaf blade explant of chrysanthemum was cultured on Murashige and Skoog(MS) medium supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid(2, 4-D) and N6-benzyl amino purine(BA). plantregeneration and root was significant respectivity according to 2, 4-D and BA concentrations, high significant respectivity at 2, 4-D×BA interaction effect, callus and plantregeneration occured in the MS medium supplemented with 0.5∼2.0mg/L 2, 4-D and 0.1∼2.0mg/L BA, and them plantregeneration occured the most medium was 0.5mg/L 2, 4-D and 0.5mg/L BA.
Plantregeneration were directry formed on the mass of light green callus induced at leaf blade explant of chrysanthemum.