토끼모델에서 저강도 맥동초음파가 골절치유에 미치는 영향

다국어 초록 (Multilingual Abstract)

The purpose of this research was to determine the effects on the healing of fibular fractures in rabbits of low-intensity pulsed ultrasound (50 mW/㎠ and 500 mW/㎠) applied for periods of 4, 14 and 24 days following fibular osteotomy. Thirty-six male Japanese white rabbits were randomly randomly divided into three groups of twelve for three treatment protocols: (1) ultrasound treatment at intensities of mW/㎠ and 500 mW/㎠ until the 4th following fibular osteotomy, (2) ultrasound treatment at intensities of 50 mW/㎠ and 500 mW/㎠ until the 14th day following fibular osteotomy, and (3) ultrasound treatment at intensities of 50mW/㎠ and 500mW/㎠ until the 24th day following fibular osteotomy. The low-intensity pulsed ultrasound was applied to only one fibular of each rabbit (these served as the experimental group). The other fibular of each rabbit served as the control group. The selection of which fibular was to be treated was made randomly. The animals were sacrificed on the 4th, 14th and 24th day after the start of ultrasound treatments. Percent of trabecular bone area and fibular radiography were carried out to compare the degree of fibular bone healing. A microscope was also used to determine any histologic changes. For statistical differences in radiological changes due to length of treatment period (4, 14 and 24 days respectively), the wilcoxon signed-ranks test was used to compare the experimental and control groups. For statistical differences in fracture healing due to differences in ultrasound intensity, radiological studies were compared using the Mann-Whitney Test. And, to compute percentage differences in areas of trabecular bone, Two-way analysis of variance (ultrasound intensity x each group) was used. Experiment results were as follows: 1. In animals sacrificed in the experimental and control groups (p>.05). However, experimental groups showed more rapid bone repair than control group. 2. Both radiographic and percent of trabecular bone area studies showed significant differences in rabbits sacrificed after 14 days. Fracture healing was significantly increased in the experimental group(p<.05) 3. In the animals sacrificed on the 24th day, histologic study showed rapid bone repair but fibular radiologic studies did not show statistical differences between the two groups(p>.05). 4. On the 14th day, bone union on radiograph was significantly more rapid in the treatment group with pulsed ultrasound of 50mW/㎠ than the group with 500mW/㎠ (p<.05). Histologic studies showed that both the 14 and 24 days groups had more rapid bone repair in animals treated with 50mW/㎠ ultrasound intensity than those treated with 500 mW/㎠ intensity. In conclusion, it has been shown that the low-intensity pulsed ultrasound has a positive effect on bone fracture healing in the early stage and the range of pulse ultrasound from 50mW/㎠ to 500mW/㎠ is effective for fracture healing. Further study is needed to investigate the influence of pulsed ultrasound on delayed union and non-union in bone fractures and also for the clinical use of low-intensity pulsed ultrasound for bone healing in humans.