RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus

다국어 초록 (Multilingual Abstract)

Drosophila E75 belongs to the nuclear receptor superfamily and plays key role in various physiological processes including ecdysone signaling. We identified an orthologue of Drosophila E75 from the Laodelphax striatellus (LsE75). Open reading frame (ORF) of the LsE75 contains 2454 nucleotides and encodes a protein of 817 amino acids. A phylogenetic analysis showed that the LsE75 clustered within the insect E75 group and was most closely related to the E75 from hemipteran insects. Quantitative PCR analysis revealed that transcription of the LsE75 was highly increased in RSV-viruliferous L. striatellus compared to that in non-viruliferous L. striatellus. To inhibit transmission of the RSV in L. striatellus using RNAi pathway, dsRNA against LsE75 (dsLsE75) was exogenously applied to the insects. As a result, transcription of RSV NS3 gene in L. striatellus ingested dsLsE75 was reduced in dose-dependent manner, suggesting that transmission of the RSV could be suppressed by silencing of LsE75 transcription in RSV-viruliferous L. striatellus

참고문헌 (Reference)

1 이주현, "Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus" 한국식물병리학회 29 (29): 330-337, 2013

2 Jones, D. T., "The rapid generation of mutation data matrices from protein sequences" 8 : 275-282, 1992

3 Yu, R., "The insect ecdysone receptor is a good potential target for RNAi-based pest control" 10 : 1171-1180, 2014

4 Hemmes, H., "The NS3 protein of Rice hoja blanca tenuivirus suppresses RNA silencing in plant and insect hosts by efficiently binding both siRNAs and miRNAs" 13 : 1079-1089, 2007

5 Segraves, W. A., "The E75 ecdysone-inducible gene responsible for the 75B early puff in Drosophila encodes two new members of the steroid receptor superfamily" 4 : 204-219, 1990

6 Carney, G. E., "The Drosophila ecdysone receptor (EcR) gene is required maternally for normal oogenesis" 154 : 1203-1211, 2000

7 Morris, L. X., "Steroid signaling within Drosophila ovarian epithelial cells sex-specifically modulates early germ cell development and meiotic entry" 7 : e46109-, 2012

8 안샛별, "Silencing of rice stripe virus in Laodelphax striatellus using virus-derived double-stranded RNAs" 한국응용곤충학회 20 (20): 695-698, 2017

9 Satoh, K., "Selective modification of rice (Oryza sativa) gene expression by rice stripe virus infection" 91 : 294-305, 2010

10 Wan, G., "Rice stripe virus counters reduced fecundity in its insect vector by modifying insect physiology, primary endosymbionts and feeding behavior" 5 : 12527-, 2015

11 Ding, S. W., "RNA-based antiviral immunity" 10 : 632-644, 2010

12 Jia, S., "RNA interference-mediated silencing of a Halloween gene spookier affects nymph performance in the small brown planthopper Laodelphax striatellus" 22 : 191-202, 2015

13 Kanakala, S., "RNA interference in insect vectors for plant viruses" 8 : 329-, 2016

14 Liu, W., "Proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein" 14 : 2229-2242, 2015

15 Zhao, W., "Organ-specific transcriptome response of the small brown planthopper toward rice stripe virus" 70 : 60-72, 2016

16 King-Jones, K., "Nuclear receptors—a perspective from Drosophila" 6 : 311-323, 2005

17 Wu, W., "Nonstructural protein NS4 of rice stripe virus plays a critical role in viral spread in the body of vector insects" 9 : e88636-, 2014

18 Ishikawa, K., "Morphological characteristics of rice stripe virus" 70 : 3465-3468, 1989

19 Jia, S., "Molecular cloning and RNA interference-mediated functional characterization of a Halloween gene spook in the white-backed planthopper Sogatella furcifera" 14 : 19-, 2013

20 Kumar, S., "MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets" 33 : 1870-1874, 2016

21 Jia, S., "Knockdown of a putative Halloween gene Shade reveals its role in ecdysteroidogenesis in the small brown planthopper Laodelphax striatellus" 531 : 168-174, 2013

22 Hogenhout, S. A., "Insect vector interactions with persistently transmitted viruses" 46 : 327-359, 2008

23 Power, A. G., "Insect transmission of plant viruses: a constraint on virus variability" 3 : 336-340, 2000

24 Zhu, J. Q., "Improvement of pest resistance in transgenic tobacco plants expressing dsRNA of an insect-associated gene EcR" 7 : e38572-, 2012

25 Siaussat, D., "Identification of steroid hormone signaling pathway in insect cell differentiation" 64 : 365-376, 2007

26 Tan, A., "Edysone receptor isoforms play distinct roles in controlling molting and metamorphosis in the red flour beetle, Tribolium castaneum" 291 : 42-49, 2008

27 Palli, S. R., "Ecdysteroid receptors and their applications in agriculture and medicine" 73 : 59-100, 2005

28 Billas, I. M. L., "Ecdysone:Structures and Functions" Springer 335-360, 2009

29 Buszczak, M., "Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila" 126 : 4581-4589, 1999

30 Ables, E. T., "Ecdysone response gene E78 controls ovarian germline stem cell niche formation and follicle survival in Drosophila" 400 : 33-42, 2015

31 Wu, W. J., "Ecdysone receptor controls wing morphogenesis and melanization during rice planthopper metamorphosis" 58 : 420-426, 2012

32 de Rosny, E., "Drosophila nuclear receptor E75 is a thiolate hemoprotein" 45 : 9727-9734, 2006

33 Bass, B. L., "Double-stranded RNA as a template for gene silencing" 101 : 235-238, 2000

34 Liu, B., "Differential proteomics profiling of the ova between healthy and rice stripe virus-infected female insects of Laodelphax striatellus" 6 : 27216-, 2016

35 Hammond, S. M., "Dicing and slicing: the core machinery of the RNA interference pathway" 579 : 5822-5829, 2005

36 Wang, L., "Cross-resistance and possible mechanisms of chlorpyrifos resistance in Laodelphax striatellus (Fallén)" 66 : 1096-1100, 2010

37 Gancz, D., "Coordinated regulation of niche and stem cell precursors by hormonal signaling" 9 : e1001202-, 2011

38 Falk, B. W., "Biology and molecular biology of viruses in the genus Tenuivirus" 36 : 139-163, 1998

39 Hemmes, H., "Binding of small interfering RNA molecules is crucial for RNA interference suppressor activity of rice hoja blanca virus NS3 in plants" 90 : 1762-1766, 2009

40 Merkling, S. H., "Beyond RNAi: antiviral defense strategies in Drosophila and mosquito" 59 : 159-170, 2013

41 Pfaffl, M. W., "A new mathematical model for relative quantification in real-time RT–PCR" 29 : e45-, 2001

42 Woodard, C. T., "A molecular mechanism for the stage specificity of the Drosophila prepupal genetic response to ecdysone" 79 : 607-615, 1994