Xanthomonas maltophilia, which can produce β-lactamase, were isolated from Galleria mellonella larvae. Medium composition for bacteria growth was optimized. As a result, the best nitrogen source was found to be yeast extract. The medium composition c...
Xanthomonas maltophilia, which can produce β-lactamase, were isolated from Galleria mellonella larvae. Medium composition for bacteria growth was optimized. As a result, the best nitrogen source was found to be yeast extract. The medium composition consisted of 5% yeast extract, 0.5% NaCl, and 0.5% KH₂PO₄. The optimum temperature for cell growth was 28℃. The culture pH approached to about 9.0 during the cell growth period regardless of initial pH ranging from 6 to 10. Meanwhile, the carbon sources such as glucose or fructose showed as decreasing trend of pH and thus inhibited the further cell growth. The specific growth rate of X. maltophilia in a 5L-fermentor was 2.6 times greater than that in a flask culture, presumably due to enhanced aeration and agitation conditions.