참비름의 라디칼 소거활성 및 콜레스테롤 급이 흰쥐에 대한 혈액 지질성분에 미치는 영향

국문 초록 (Abstract)

참비름의 in vitro 라디칼 소거활성 및 콜레스테롤 급이 흰쥐에 보충 급이할 경우 혈액 중 지질 성분의 함량에 미치는 영향을 분석하였다. 참비름 물 및 80% 에탄올 추출물의 ABTS, DPPH 및 nitric oxide 라디칼 소거활성은 물 추출물보다 에탄올 추출물에서 유의적으로 높았다. 콜레스테롤을 급이한 대조군에 비해 5%(HCA1) 및 10%(HCA2)의 참비름 분말 급이 시 혈청 총 지질, 총 콜레스테롤 함량, 동맥경화 지수 및 심혈관질환 위험지수는 시료의 첨가량이 증가됨에 따라 유의적으로 감소하였다. 중성지방은 HCA2군에서만 유의적인 감소를 보였으며, HDL-콜레스테롤은 대조군에 비해 참비름 분말 급이 시 증가하였으나, 첨가량에 따른 유의차는 없었다. 혈중 GPT 및 γ-GTP 활성은 대조군에 비하여 참비름 분말 급이군에서 유의적으로 감소하였다. 혈중 지질과산화물 함량도 같은 경향이었다. 따라서 참비름은 식이성 콜레스테롤에 의한 질환의 조절에 유효할 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

To evaluate of biological activity of Chambirum (Amaranthus lividus) in vitro and in vivo, we investigated the free radical scavenging activity of its extracts in vitro and the effect of lyophilized powder on the serum lipid profile of rats fed cholesterol. ABTS, DPPH, and NO radical scavenging activities were tested from water and 80% ethanol extracts of Chambirum, and biological activities of the ethanol extracts were significantly higher than the water extracts. The total lipid and total cholesterol content of serum, atherogenic index (AI), and cardiac risk factor (CRF) were decreased significantly for the groups fed with a 5% and 10% supplement of Chambirum powder (HCA1 and HCA2) in comparison with the group fed cholesterol (HC). Triglyceride content decreased drastically in the HCA2 group, while its content was not decreased in the other group. HDL-cholesterol content was elevated in the HCA1 and HCA2 groups, but was not significantly different to the supplemented amount of Chambirum powder. GPT and γ-GTP activities were decreased significantly in the groups fed with Chambirum powder compared to the HC group. And the content of the lipid peroxide level was the same trend. Therefore, these results give evidence that Chambirum might be useful in the control of induced disorders by dietary cholesterol and/or lipids.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 문헌

참고문헌 (Reference)

1 황의경, "퀘르세틴 첨가가 고지방 및 고콜레스테롤 식이 랫드의 혈청 성분 농도의 변화에 미치는 영향" 한국임상수의학회 26 (26): 413-418, 2009

2 오영숙, "참비름 추출물에서 항균성 물질의 분리 및 동정" 한국미생물·생명공학회 33 (33): 123-129, 2005

3 김미주, "쇠비름의 무기물 함량 및 항산화 활성" 한국생명과학회 21 (21): 1393-1400, 2011

4 김애정, "뽕잎분말이 고콜레스테롤 식이 투여 흰쥐의 지질대사에 미친 영향" 한국식품과학회 37 (37): 636-641, 2005

5 박현숙, "맥아와 한약재추출물이 고지방 식이를 섭취시킨 흰쥐의 혈장지질 및 혈당농도에 미치는 영향" 대한본초학회 24 (24): 15-21, 2009

6 이재준, "대두와 된장분말이 고콜레스테롤식이를 급여한 흰쥐의 콜레스테롤 농도 및 항산화 활성에 미치는 영향" 한국생명과학회 20 (20): 1134-1142, 2010

7 신미경, "녹차 건분이 고지방식이 급여에 의한 흰쥐의 지질 농도에 미치는 영향" 한국식생활문화학회 18 (18): 226-234, 2003

8 황의경, "나린진이 고지방 및 고콜레스테롤 식이 랫드의 혈청 성분 농도의 변화에 미치는 영향" 한국임상수의학회 26 (26): 231-237, 2009

9 강상모, "고지혈증 유발 흰쥐에 있어서 생식의 건강개선효과" 한국식품영양과학회 32 (32): 906-912, 2003

10 곽충실, "고지방고콜레스테롤식이를 섭취한 흰쥐에서 식물성식품의혼합물 첨가로 인한 체내 항산화능 증가 효과" 한국영양학회 38 (38): 352-363, 2005

11 Shin JH, "The formation and inhibition of N-nitrosamine in common Korean foods" Gyeongsang National University 2002

12 Haglund O, "The effect of oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in man supplemented with vitamin" 121 : 165-172, 1991

13 Holmberg D, "The effect of amaranth treatment on some kidney and liver enzymes in the rat" 46 : 257-260, 1978

14 Cho KJ, "Purification and characterization of antiviral protein (AAP29) from the leaves of Amaranthus mangostanus" 38 : 528-533, 1995

15 Cheung PCK, "Plasma and hepatic cholesterol levels and fecal neutral sterol excretion are altered in hamsters fed straw mushroom diets" 128 : 1512-1516, 1998

16 Czerwiski J, "Oat (Avena sativa L.) and amaranth (Amaranthus hypochondriacus) meals positively affect plasma lipid profile in rats fed cholesterol-containing diets" 15 : 622-629, 2004

17 Kim YH, "Isolation on rutin from Amaranthus mangostanus" 31 : 249-251, 2000

18 Ozsoy N, "In vitro antioxidant activity of Amaranthus lividus L" 116 : 867-872, 2009

19 Ashok Kumar BS, "In vitro alpha-amylase inhibition and in vivo antioxidant potential of Amaranthus spinosus in alloxan-induced oxidative stress in diabetic rats" 18 : 1-5, 2011

20 Hee Sun Song, "In Vitro Antioxidant Activity Profiles of β-Glucans Isolated from YeastSaccharomyces cerevisiae and Mutant Saccharomyces cerevisiae IS2" 한국식품과학회 15 (15): 437-440, 2006

21 Frings CS, "Improved determination of total serum lipids by the sulfo- phospho-vanillin reaction" 18 : 763-764, 1972

22 Zeashan H, "Hepatoprotective and antioxidant activity of Amaranthus spinosus against CCl4 induced toxicity" 125 : 364-366, 2009

23 Friedewald WT, "Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge" 18 : 499-502, 1972

24 Cha JY, "Effects of water-soluble extract from leaves of Morus alba and Cudrania tricuspidata on the lipid peroxidation in tissues of rats" 29 : 531-536, 2000

25 Mantha SV, "Effects of probucol on hypercholesterolemia-induced changes in antioxidant enzymes" 58 : 503-509, 1996

26 Soo-Jung Lee, "Effects of Portulaca oleracea Powder on the Lipid Levels of Rats Fed a Hypercholesterolemia Inducing Diet" 한국식품영양과학회 16 (16): 202-209, 2011

27 Grageta H, "Effect of amaranth and oat bran on blood serum and liver lipids in rats depending on the kind of dietary fats" 43 : 114-117, 1999

28 Repo-Carrasco-Valencia R, "Dietary fiber and other functional components in two varieties of crude and extruded kiwicha (Amaranthus caudatus)" 49 : 219-224, 2009

29 Hertog MG, "Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study" 342 : 1007-1011, 1993

30 Wu JH, "Coronary artery disease risk predicted by plasma concentrations of high- density lipoprotein cholesterol, apolipoprotein AI, apolipoprotein B, and lipoprotein(a) in a general Chinese population" 39 : 209-215, 1993

31 Plate Andrea YA, "Cholesterol-lowering effect of extruded amaranth (Amaranthus caudatus L.) in hypercholesterolemic rabbits" 76 : 1-6, 2002

32 Glowinska B, "Cardiovascular risk factors in children with obesity, hypertension an diabetes: lipoprotein(a) levels and body mass index correlate with family history of cardiovascular disease" 161 : 511-518, 2002

33 Lee SJ, "Biological activities of extracts from Bireum (Amaranthus lividus)" 2010

34 Fasuyi AO, "Bio-nutritional evaluations of three tropical leaf vegetables (Telfairia occidentalis, Amaranthus cruentus and Talinum triangulare) as sole dietary protein sources in rat assay" 103 : 757-765, 2007

35 Russell DW, "Bile acid biosynthesis" 31 : 4737-4749, 1992

36 Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobabituric acid reaction" 95 : 351-358, 1979

37 Yagi K, "Assay for blood plasma or serum, In Method in Enzymology, Vol 105" Academic Press 328-331, 1984

38 Kim HK, "Antioxidative anti-diabetic effects of amaranth (Amarnthus esculantus) in streptozotocin-induced diabetic rats" 24 : 195-199, 2006

39 Pacifico S, "Antioxidant evaluation of polyhydroxylated nerolidols from redroot pigweed (Amaranthus retroflexus) leaves" 41 : 1665-1671, 2008

40 Blois MS, "Antioxidant determination by the use of a stable free radical" 181 : 1199-1200, 1958

41 Gupta M, "Antioxidant and free radical scavenging activities of Ervatamia coronaria Stapf. leaves" 2 : 119-126, 2004

42 Sexena R, "Antioxidant activity of commonly consumed plant foods of India: contribution of their phenolic content" 58 : 250-260, 2007

43 Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay" 26 : 1231-1237, 1999

44 Sangameswaran B, "Anti-diabetic, anti- hyperlipidemic and spermatogenic effects of Amaranthus spinosus Linn. on streptozotocin-induced diabetic rats" 62 : 79-82, 2008