營養膜細胞의 植物凝集素 結合部에 關한 硏究 = A Study on Lectin Receptor of Human Trophoblasts

다국어 초록 (Multilingual Abstract)

Lectins are proteins and glycoproteins that bind specifically to mono-or oligosaccharides and are found primarily in plants. And extensive use of lectins has been made as cytochemical membrane markers through their fluorescein isothiocyanate or horseradish peroxidase conjugates.
Biotinyl derivatives of commercially available plant lectins-Concanavalin A (Com A), Peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA I), Soybean agglutinin (SBA), Dolichos biflorus agglutinin I (DBA), Wheat germ agglutinin (WGA), Ulex europaeus agglutinin I (UEA I), and Pinellia ternate agglutinin (PTA) purified and biotinylated at this institute were applied (bound) to sections of formalin fixed and paraffin embedded clinical samples of young and term placenta hydatidiform mole, invasive mole and choriocarcinoma, and the reacted with avidin-biotinylated peroxidase (Standard Vectastain ABC Kit) and diaminobenzidine solution for the visualization of bound lectin.
The most interesting staining was observed with WGA which revealed strong positive reactions both in syncytic-and cytotrophoblasts of hydatidiform mole, invasive mole and choriocarcinoma, contrary to normal young chorionic villi which showed linear positive reaction only along the brush border of syncytiotrophoblast. In term placenta, only weak positive reaction was noted along the brush border of syncytiotrophoblast.
Con-A and PTA were unique in providing strong positive reaction of syncytiotrophoblast in diffuse cytoplasmic pattern throughout the normal and pathologic trophoblasts. Other lectins gave no visualization.
The above results suggest that cytotrophoblast in trophoblastic diseases exhibits inhibiting sugar to WGA ((beta-Nacetyl-aminy-), Sialic acid) which is absent in normal cytotrophoblast. Additional visualizations were positive reactions of WGA in red blood cells and vascular luminal surface, PTA in tissue mast cells in the myometrium, and WGA, PNA, Con-A and PTA along the glandular lumina of the endometrium.