간접형광항체반응에 의한 인체낭미충증의 혈청학적 진단에 관한 연구 = Serodiagnosis of human cysticercosis using sectioned antigen of Taenia solium cysticerci in an IFAT

다국어 초록 (Multilingual Abstract)

The indirect fluorescent antibody test (IFAT) for cysticercosis was accomplished with the sectioned cyst wall tissue antigen which was made with the cyst in the fourth ventricle of human brain. The antigen was revealed on the tegument, nuclear layer and internal surface of the bladder wall with FITC conjugated IgG fraction goat antihuman immunoglobulins. Total 236 sera were tested which were obtained from 24 cases of confirmed cerebral or dermal cysticercosis patients, 33 cases of suspected cysticercosis, 50 cases of intestinal or tissue invading larval cestodiasis, 50 cases of trematodiasis, 14 cases of nematodiasis or elevated eosinophilia and 65 cases of non-infected control sera. The serum titer of 1:16 or over was regarded as positive. Twenty three out of twenty four (95.8%) sera from confirmed cysticercosis patients were positive. Serum titer of 65 non-infected controls showed 1:8 or below. Twelve cases out of thirty three (36.4%) suspected cases of cysticercosis were revealed as positive. And cross reactions were observed in taeniasis solium (40.0%), taeniasis saginata (13.3%), diphyllobothridiasis laturn (11.8%) and lonorchiasis sinensis (2.8%). The mean titer of cerebral cysticercosis (7.60 in log₂) was higher than those of mixed (5.61 in log₂) and dermal cysticercosis (5.44 in log₂). The titers of cerebral cysticercosis patient's sera who were treated with praziquantel were not changed at least within six months in four cases out of seven followed up cases. In four to five year after treatment with praziquantel it showed decreased or negatively converted serum titers.
Conclusively, it was indicated that indirect fluorescent antibody test using the sectioned antigen of Taenia solium cysticerci was applicable to the diagnosis of cysticercosis.