Sorting CD4+ T Cells in Blood by Using Magnetic Nanoparticles Coated with Anti-CD4 Antibody

다국어 초록 (Multilingual Abstract)

We used Fe3O4 magnetic nanoparticles (MNPs) which are coated with antiCD4 monoclonal
antibody to bind selectively onto membranes of CD4+ T cells (hereafter antiCD4-MNPs). The
antiCD4-MNPs were prepared through direct covalent interaction between the carboxyl group of
the antiCD4 antibody and the amino group of amino-modified MNPs. The antiCD4-MNPs were
mixed with human blood cells, followed by bursting the red blood cells with hypotonic buffer; then,
the antiCD4-MNPs coated cells were separated by using a magnet. We observed the number of cells
bound with magnetite clusters and particles. When fluorescence isothiocyanate labeled antiCD4-
MNPs was used to observe the CD4+ T cells, the fluorescent intensity was improved by about two times compared to that when cells were labeled with the antiCD4 antibody only. This is a potential method to sort helper CD4+ T cells for observation under conventional microscopes.

다국어 초록 (Multilingual Abstract)

We used Fe3O4 magnetic nanoparticles (MNPs) which are coated with antiCD4 monoclonal
antibody to bind selectively onto membranes of CD4+ T cells (hereafter antiCD4-MNPs). The
antiCD4-MNPs were prepared through direct covalent interaction between the carboxyl group of
the antiCD4 antibody and the amino group of amino-modified MNPs. The antiCD4-MNPs were
mixed with human blood cells, followed by bursting the red blood cells with hypotonic buffer; then,
the antiCD4-MNPs coated cells were separated by using a magnet. We observed the number of cells
bound with magnetite clusters and particles. When fluorescence isothiocyanate labeled antiCD4-
MNPs was used to observe the CD4+ T cells, the fluorescent intensity was improved by about two times compared to that when cells were labeled with the antiCD4 antibody only. This is a potential method to sort helper CD4+ T cells for observation under conventional microscopes.

참고문헌 (Reference)

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