담죽엽 추출물의 혈관이완 기전에 대한 연구 = Effect of Lophatherum gracile on the mechanism of vasorelaxation in thoracic aorta

다국어 초록 (Multilingual Abstract)

The vasorelaxant effect of an extract of Lophatherum gracile Brongn (ELB) and its possible action mechanism were ascertained in aortic tissues isolated from rats. ELB relaxed endothelium-intact thoracic aorta in a dose-dependent manner. However, the induced vascular relaxation was abolished by removal in endothelium of the thoracic aorta. Pretreatment of endothelium-intact vascular tissues with $N^G$-nitro-L-arginine methyl ester (L-NAME) or 1H-[1,2,4]-oxadiazole-[4,3-$\alpha$]-quinoxalin-1-one (ODQ) significantly inhibited vascular relaxation induced by ELB. Moreover, ELB significantly increased cGMP production in aortic tissues, which was blocked by pretreatment with L-NAME or ODQ. The vasorelaxant effect of ELB was attenuated by tetraethylammonium (TEA), and glibenclamide. ELB-induced vasorelaxation was not blocked by atropine, propranolol, indomethacin, verapamil, and diltiazem. Taken together, the present study demonstrates that ELB dilates vascular smooth muscle via an endothelium-dependent NO-cGMP signaling pathway, which may be at least in part related with the function of $K^+$ channels.

참고문헌 (Reference)

1 이상인, "한방임상처방학(韓方臨床處方 學): 방제구성의 이론과 실제" 영림사 361-384, 1998

2 신영기, "주치의와 환자가 함께 보는 교양서 고혈압" 계축문화사 40-, 2000

3 김창민, "완벽 중약대사전. 제2권" 정담 881-888, 1999

4 최윤정, "서울 및 경기지역 노인의 건강자가평가에 따른 기능적 건강 및 영양위험 평가" 한국영양학회 37 (37): 223-235, 2004

5 생약학교재편찬위원회, "생약학" 동명사 47-, 1998

6 권년수, "산화질소 생성의 생화학" 41-45, 1995

7 E Braunwald, "국역 Harrison's principles of internal medicine" 도서출판 MIP 1456-1460, 2003

8 Ko FN, "Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta" 1115 : 69-74, 1991

9 Levi R, "Therapies for perioperative hypertension: Pharmacodynamic consideration" 37 : 16-19, 1993

10 Furchgott RF, "The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine" 288 : 373-376, 1980

11 Sharma NR, "Substance P-induced calcium entry in endothelial cells is secondary to depletion of intracelluar stores" 268 : 962-973, 1995

12 Kim NJ, "Studies on the development of antihyperlipidemic drugs from Oriental Herbal Medicines (I)－Antihyperlipidemic activities of Oriental herbal medicines" 30 : 368-376, 1999

13 Furchgott RF, "Studies on relaxation of rabbit aorta by sodium nitrite: the basis for the proposal that the acid-activatable inhibitory factor from retractor penis is inorga nicnitrite and the endothelium-derived relaxing factor is nitric oxide. in: Vasodilation: Vascular Smooth Muscle, Peptides, and Endothelium" Raven Press 401-414, 1988

14 Guerrero MF, "San Roman, L.Quercetin 3, 7-dimethyl ether:a vasorelaxant flavonoid isolated from Croton schiedeanus Schlecht" 54 : 1373-1378, 2002

15 Kim SZ, "Presence and biological activity of C-type natriuretic peptide-dependent guanylate cyclase-coupled receptor in the penile corpus cavernosum" 159 : 1741-1746, 1998

16 Loeb AL, "Peach MJ. Endothelium-derived relaxing factor release associated with increased endothelial cell inositol triphosphate and intracellular calcium" 62 : 36-40, 1988

17 Okabe K, "Features of 4-aminopyridine sensitive outward current observed in single smooth muscle cells from the rabbit pulmonary artery" 409 : 561-568, 1987

18 Martin N, "Experimental cardiovascular depressant effect of garlic dialysate" 37 : 145-153, 1992

19 Rubanyi GM, "Endothelium-derived relaxing and contracting factors" 46 : 27-36, 1991

20 Vanhoutte Paul M, "Endothelium-dependent hyperpolarizations: the history" 49 : 503-508, 2004

21 Hau C, "Endothelial dysfunction in cardiovascular diseases; the role of oxidant stress" 87 : 840-844, 2000

22 Park PS, "Effects of Dioscorea batatas and Gastrodiae rhizoma on lipid composition changes of liver, brain and kidney in rats" 9 : 188-194, 1999

23 Lemos VS, "Diocleinanew nitric oxide- and endothelium-dependent vasodilator flavonoid" 386 : 41-46, 1999

24 MacNaul KL, "Differential of iNOS and cNOS mRNA in human vascular smooth muscle cells and endothelial cells under normal and inflammatory conditions" 196 : 1330-1334, 1993

25 Hutcheson IR, "Central role of intracellular calcium stores in acute flowand agonist-evoked endothelial nitric oxide release" 122 : 117-125, 1997

26 Nevala R, "Calcium-sensitive potassium channel inhibitors antagonize genistein-and daidzein-induced arterial relaxation in vitro" 69 : 1407-1417, 2001

27 Van Breemen C, "Ca2+ regulation of vascular smooth muscle" 2746-2751, 1986

28 Major Disease and Health-related behaviors in Korea, "An In depth analysis of 2001 National Health and Nutrition Examination survey" Ministry of Health and Welfare 76-, 2003

29 Meng W, "Ach dilates pial arterioles in endothelial and neuronal NOS knockout mice by NO-dependent mechanisms" 271 : 1145-1150, 1996