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RISS
A simple, rapid and high sensitive method for the determination of prostaglandin F_2α (C_20H_34O_5) is described. 1-bromoacetylpyrene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography. PG F_2α w...
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RISS 인기검색어
1-Bromoacetylpyrene 誘導體化劑를 利用한 carboxyl基 含有成分의 分析에 관한 硏究(Ⅳ) : Prostaglandin F_2α의 HPLC에 依한 定量 Determination of prostaglandin F_2α by HPLC = Studies on the HPLC Determination of Carboxyl Group using 1-bromoacetylpyrene as a Derivatizing Reagent(Ⅳ)
한글로보기https://www.riss.kr/link?id=A19599868
- 저자
- 발행기관
- 학술지명
- 권호사항
-
발행연도
1989
-
작성언어
Korean
-
KDC
409.000
-
자료형태
학술저널
-
수록면
7-12(6쪽)
- 제공처
- 소장기관
-
0
상세조회 -
0
다운로드
부가정보
다국어 초록 (Multilingual Abstract)
A simple, rapid and high sensitive method for the determination of prostaglandin F_2α (C_20H_34O_5) is described.
1-bromoacetylpyrene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography.
PG F_2α was derivatized quantiatively into fluorescent compound by reacting with 1-bromoacetylpyrene in the presence of 18-crown-6 in acetonitrile.
The derivative was separated on a normal phase column in isocratic elution mode using the secondary mixture of chloroform and tetrahydrofuran as the mobile phase.
The effluent was monitored by fluorometer.
The calibration plots for the peak heights versus concentrations of PG F_2α were observed to he linear.
1-bromoacetylpyrene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography.
PG F_2α was derivatized quantiatively into fluorescent compound by reacting with 1-bromoacetylpyrene in the presence of 18-crown-6 in acetonitrile.
The derivative was separated on a normal phase column in isocratic elution mode using the secondary mixture of chloroform and tetrahydrofuran as the mobile phase.
The effluent was monitored by fluorometer.
The calibration plots for the peak heights versus concentrations of PG F_2α were observed to he linear.
A simple, rapid and high sensitive method for the determination of prostaglandin F_2α (C_20H_34O_5) is described.
1-bromoacetylpyrene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography.
PG F_2α was derivatized quantiatively into fluorescent compound by reacting with 1-bromoacetylpyrene in the presence of 18-crown-6 in acetonitrile.
The derivative was separated on a normal phase column in isocratic elution mode using the secondary mixture of chloroform and tetrahydrofuran as the mobile phase.
The effluent was monitored by fluorometer.
The calibration plots for the peak heights versus concentrations of PG F_2α were observed to he linear.
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