Mercury Ions Mediated Phosphorus Containing Carbon Dots as Fluorescent Probe for Biothiols Screening

다국어 초록 (Multilingual Abstract)

In this study, we report the mercury ions (Hg2+) mediated phosphorus-containing carbon dots (PCDs) as a selective "off–on" fluorescence probe for glutathione (GSH), cysteine (Cys) and homocysteine (Hcys). PCDs obtained by hydrothermal reaction are sensitive to Hg2+ ions and its fluorescence can be significantly quenched owing to the electron transfer from the lowest unoccupied molecular orbital (LUMO) of PCDs to Hg2+. Interestingly, the weak fluorescence of Hg2+-mediated PCDs could be gradually recovered with the addition of GSH, Cys and Hcys. This can be attributed to the formation of Hg2+–S complex due to the super affinity of Hg2+–sulfydryl bond. The formation of Hg2+–S complex extremely reduces the oxidation ability of Hg2+ that inhibits the electron transfer from LUMO of PCDs to Hg2+ and re-opens the native electron transition from LUMO to the highest occupied molecular orbital (HOMO) of PCDs. Thus, the green fluorescence of PCDs is switched on. Furthermore, the present Hg2+-mediated PCDs assay exhibits a high selectivity for GSH, Cys and Hcy and has been successfully used to detect the total biothiols content in human urine samples.

참고문헌 (Reference)

1 J. X. Dong, 281 : 473-, 2016

2 N. Zhang, 42 : 214-, 2013

3 B. Y. Han, 81 : 5569-, 2009

4 K. S. Park, 45 : 65-, 2013

5 J. Gu, 68 : 27-, 2015

6 X. Zhai, 48 : 7955-, 2012

7 F. Yan, 251 : 753-, 2017

8 A. Gupta, 81 : 465-, 2016

9 V. Borse, 248 : 481-, 2017

10 Z. J. Li, 68 : 668-, 2015