사염화탄소를 투여한 흰쥐에서 녹차 추출물이 간기능 개선 및 항섬유화에 미치는 영향 = Effects of Water Extracts of Green Tea on Hepatic Functional Improvement and Anti-ibrosis in Ratsadministered Carbon Tetrachloride

국문 초록 (Abstract)

사염화탄소를 투여한 흰쥐에게 녹차 추출물을 급여하여 간기능 개선 및 항섬유화에 미치는 영향을 연구하였다.
1. 식이 섭취량, 체중 증가량 및 식이효율은 정상군에 비하여 대조군과 녹차 추출물 급여군이 유의적인 차이는 없었다.
2. 간 무게와 체중 당 간 무게의 비는 대조군에 비하여 녹차 추출물 급여군이 낮았으나 유의적인 차이는 없었다.
3. 혈청의 AST, ALT 활성 변화는 대조군에 비하여 녹차 추출물 급여군이 유의적으로 낮았고(p < 0.01), ALP 활성변화는 대조군에 비하여 녹차 추출물 급여군인 Ga군에서는 낮았으나 유의적인 차이는 없었고, Gb군에서는 유의적으로 낮았다(p < 0.05). HDL-cholesterol 수준은 대조군에 비하여 녹차 추출물 급여군이 높았으나 유의적인 차이는 없었고, LDL-cholesterol 수준은 대조군에 비하여 녹차 추출물 급여군이 낮았으나 유의적인 차이는 없었다.
4. 간 조직의 MDA 함량은 대조군에 비하여 녹차 추출물 급여군이 유의적으로 낮았고(p < 0.05), hydroxyproline 함량은 대조군에 비하여 녹차 추출물 급여군이 낮았으나 유의적인 차이는 없었다.
5. 간 조직의 형태학적 변화는 대조군에 비하여 녹차 추출물 급여군이 콜라겐의 증가와 지방변성과 지방적이 약하게 나타났다.
결론적으로 녹차 추출물은 흰쥐에 사염화탄소를 투여하여 간의 지질과산화에 의한 독성 유도시 혈청의 AST, ALT, ALP, HDL-cholesterol, LDL-cholesterol, 간 조직의 MDA와 hydroxyproline의 개선 경향 및 콜라겐 축적 억제, 간 조직 손상 보호를 통해 간기능 개선과 항섬유화 효과가 있는 것으로 판단되므로 이에 따른 보다 많은 연구가 이루어져야 할 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

This study examined the hepatic functional improvement and anti-fibrotic effects of the water extracts of green tea. Male Sprague-Dawley rats were divided into four groups (normal, control, and two experimental subgroups(Ga and Gb)) and observed for 3 weeks. Liver fibrosis was induced in the rats by carbon tetrachloride (CCl₄) administration, with the exception of the normal group. With the exception of the normal and control group, the two experimental subgroups were fed water extracts of green tea. No significant differences were observed in the food intake, body weight gain or food efficiency ratio between the normal group and experimental group. The liver weight and the liver weight to body weight ratio decreased in the experimental group compared to the control group, but the difference was not significant. The serum levels of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase was significantly lower in the experimental group than the control group. The levels of high density lipoprotein-cholesterol in serum increased in the experimental group compared to the control group, but the difference was not significant. The levels of low density lipoprotein-cholesterol in the serum decreased in the experimental group compared to the control group, but the difference was not significant. The malondialdehyde levels in the liver tissue were significantly lower in the experimental group than the control group. No significant differences in hydroxyproline in the liver tissue were observed between the experimental group and control group. Regarding the liver histology, weaker inflammation and fibrosis were observed in the experimental group compared to the control group. In conclusion, the water extracts of green tea help hepatic cells keep maintain their functions, restraining and protecting the liver from the impairments caused by CCl₄ administration, and can be effective as anti-fibrotic agents.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 적요
• 참고문헌

참고문헌 (Reference)

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