열처리 살균 유산균을 이용한 알코올성 간독성 억제 효과

국문 초록 (Abstract)

사균 유산균의 알코올성 급성 간 질환과 알코올성 숙취해소에 대한 효과를 확인하고자 하였다. 조직학적 조사에서 EtOH 처리군 이외에 변화는 없었으나, 간세포에서 지방립의 변화에서 EtOH와 사균 유산균분말 고농도투여군(P＜ 0.01)에서 유의성을 보였다. 그리고 간모세혈관확장 score에서도 유사하게 양성대조군(P＜0.01)과 EtOH와 사균 유산균분말 고농도투여군(P＜0.05)에서 유의적인 완화를 보였다. 간 손상의 지표인 AST 활성의 경우 EtOH와 함께 사균 유산균 분말 고농도투여군(P＜0.001)에서 유의적인 감소를 보였고, ALT의 활성에서도 EtOH와 함께 사균 유산균 분말고농도투여군(P＜0.01)에서 억제시켰다. 한편 염증지표로서 cytokine 발현을 보았는데, IL-1β mRNA 발현이 모든 처리군에서(P＜0.001) 매우 유의하게 억제되었지만 IL-6는 사균 유산균군(P＜0.05)에서만 억제되었다. EtOH 투여 전후의 EtOH와 acetaldehyde양의 변화에서 EtOH 투여 전후 EtOH 감소 효과가 사균 유산균군에서 감소 효과를 보였다(P＜0.001). 한편 ADH와 ALDH 활성은 EtOH 투여와 상관없이 사균 유산균군에서 매우 유의하게 증가시키는 것을 확인하였다(P＜0.001). 따라서 사균 유산균이 알코올성 급성간 질환들에 유의한 효과를 나타내는 것을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

The purpose of this study was to examine the effects of heat-killed lactic acid bacteria on acute alcoholic liver disease and alcoholic hangover. Histologic analysis exhibited no change except in the ethanol-treated group, but the EtOH＋heat-killed Lactobacillus plantarum high dose-treated group (P＜0.01) showed a significant change in the fatty granules in hepatocytes. Similarly, the positive control group (P＜0.01) and the EtOH＋HLPH (heat-killed L. plantarum high dose) group (P＜0.05) showed significant difference in the hepatic telangiectasis score. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were significantly decreased in the EtOH＋HLPH group (P＜0.001 and P＜0.01 respectively). Inhibition of inflammation was assessed through cytokine expression. IL-1β mRNA expression was significantly inhibited in all treatment groups, but IL-6 was inhibited only in the heat-sterilized lactic acid bacteria group (P＜0.05). While measuring the changes in the amount of ethanol and acetaldehyde in the blood before and after ethanol administration, it was observed that the heat-killed lactic acid bacteria group effectively decreased after ethanol administration (P＜0.001). In addition, it was confirmed that alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities were significantly increased in the heat-killed lactic acid bacteria group (P＜0.001). Therefore, it was observed that heat-treated and sterilized lactic acid bacteria like live bacteria had a significant effect on acute alcoholic liver disease.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• REFERENCES

참고문헌 (Reference)

1 이희섭, "유산균 발효 마늘 추출물의 oleic acid로 유도된 비알코올성 지방간에 대한 개선 효과" 한국식품과학회 46 (46): 762-768, 2014

2 기주영, "알코올 대사 효소들의 유전적 다형성이 음주 행태 및 간경변증 발생에 미치는 영향" 대한간학회 9 (9): 89-97, 2003

3 조유란, "아토피유발인자 억제효과를 증대하는 Lactococcus lactis의 배양방법" 한국미생물·생명공학회 40 (40): 310-318, 2012

4 안희영, "발효누에분말 투여가 orotic acid 유발 흰쥐에 미치는 지방간 개선 효과" 한국생명과학회 23 (23): 789-795, 2013

5 최지현, "발효 전후 구기자열매 추출물의 ADH 및 ALDH 활성인자 분리 및 특성" 한국생물공학회 31 (31): 134-143, 2016

6 서민정, "발효 꾸지뽕(Cudrania tricuspidata) 열매 추출물이 마우스 비장세포의 cytokine 생성에 미치는 영향" 한국생명과학회 23 (23): 682-688, 2013

7 김민수, "면역결핍 모델에서 β-1,3/1,6-glucan과 유산균을 이용한 in vivo 면역 활성 조절 효과" 한국식품저장유통학회 24 (24): 1158-1167, 2017

8 Kim WJ, "Understanding of killed lactic acid bacteria as a probiotics" 4 : 115-122, 2018

9 Tottmar SOC, "The subcellular distribution and properties of aldehyde dehydrogenase in rat liver" 135 : 577-586, 1973

10 O’Hara AM, "The gut flora as a forgotten organ" 7 : 688-693, 2006

11 Niu HL, "The efficacy and safety of probiotics in patients with irritable bowel syndrome: Evidence based on 35randomized controlled trials" 75 : 116-127, 2020

12 Maruyama M, "The effects of non-viable Lactobacillus on immune function in the elderly: a randomised, double-blind, placebo-con-trolled study" 67 : 67-73, 2016

13 Choi JT, "The effect of Schizandrae Fructus extract on alcohol fermentation and enzyme activities of Saccharomyces cerevisiae" 38 : 278-282, 1995

14 Catt H, "Systematic review:outcomes and adverse events from randomised trials in Crohn’s disease" 49 : 978-996, 2019

15 Baldi E, "Serum malondialdehyde and mitochondrial aspartate aminotransferase activity as markers of chronic alcohol intake and alcoholic liver disease" 25 : 429-432, 1993

16 Rao RK, "Protection and restitution of gut barrier by probiotics: nutritional and clinical implications" 9 : 99-107, 2013

17 Robert Hendler, "Probiotics in the Treatment of Colorectal Cancer" MDPI AG 5 (5): 101-, 2018

18 Zipporah Iheozor-Ejiofor, "Probiotics for maintenance of remission in ulcerative colitis" Wiley 2020

19 Mallon PT, "Probiotics for induction of remission in ulcerative colitis" 3 : CD005573-, 2007

20 Gu Z, "Probiotics for alleviating alcoholic liver injury" 2019

21 Lorenzo Drago, "Probiotics and Colon Cancer" MDPI AG 7 (7): 66-, 2019

22 Chen MF, "Pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces the pathogenicity of influenza and enterovirus 71 infections" 10 : 215-227, 2017

23 Khoder G, "Potential role of probiotics in the management of gastric ulcer" 12 : 3-17, 2016

24 Gu YH, "Pharmaceutical production of anti-tumor and immunepotentiating Enterococcus faecalis-2001 β-glucans: Enhanced activity of macrophage and lymphocytes in tumor-implanted mice" 18 : 653-661, 2017

25 Law K, "Nonalcoholic fatty liver disease" 14 : 591-604, 2010

26 Baker RG, "NF-κB, inflammation, and metabolic disease" 13 : 11-22, 2011

27 Sang LX, "Live and heat-killed probiotic: effects on chronic experimental colitis induced by dextran sulfate sodium (DSS) in rats" 8 : 20072-20078, 2015

28 Junhua Jin, "Live and heat-killed cells of Lactobacillus plantarum Zhang-LL ease symptoms of chronic ulcerative colitis induced by dextran sulfate sodium in rats" Elsevier BV 71 : 103994-, 2020

29 de Oliveira GLV, "Intestinal dysbiosis and probiotic applications in autoimmune diseases" 152 : 1-12, 2017

30 최예진, "Immunomodulatory effects of seven viable and sonicated Lactobacillus spp. and anti-bacterial activities of L. rhamnosus and L. helvetilus" 한국미생물학회 55 (55): 392-399, 2019

31 Choi EJ, "Heat-killed Enterococcus faecalis EF-2001 ameliorates atopic dermatitis in a murine model" 8 : 146-, 2016

32 Ting WJ, "Heat killed Lactobacillus reuteri GMNL-263 reduces fibrosis effects on the liver and heart in high fat diet-hamsters via TGF-β suppression" 16 : 25881-25896, 2015

33 FAO, "Guidelines for the evaluation of probiotics in food" FAO/WHO 2002

34 Luz María Rocha-Ramírez, "Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro" MDPI AG 8 (8): 79-, 2020

35 Park SC, "Ethanol oxidation is accelerated by augmentation of malate-aspartate shuttle with aspartate" 25 : 137-143, 1993

36 Sandra Kluijfhout, "Efficacy of the Probiotic Probiotical Confirmed in Acute Gastroenteritis" 대한소아소화기영양학회 23 (23): 464-471, 2020

37 Inoue Y, "Effects of oral administration of Lactobacillus acidophilus L-92 on the symptoms and serum cytokines of atopic dermatitis in Japanese adults: a doubleblind, randomized, clinical trial" 165 : 247-254, 2014

38 Hara K, "Effect of heat-killed lactic acid bacteria Enterococcus faecalis on methicillin-resistant Staphylococcus aureus (MRSA) infected mice" 67 (67): 32-43, 2018

39 Sugahara H, "Differences between live and heat-killed bifidobacteria in the regulation of immune function and the intestinal environment" 8 : 463-472, 2017

40 Anisowicz A, "An NF-kappa B-like transcription factor mediates IL-1/TNF-alpha induction of gro in human fibroblasts" 147 : 520-527, 1991

41 Jae-Young Cha, "Alcoholic Hepatotoxicity Suppression in Alcohol Fed Rats by Glutathione-enriched Yeast FF-8 Strain" 한국식품과학회 18 (18): 1411-1416, 2009

42 Racker E, "Alcohol dehydrogenase from baker’s yeast" 1 : 500-503, 1955

43 Shumate RP, "A study of the metabolism rates of alcohol in the human body" 14 : 83-100, 1967