In Vitro Cytotoxic Effect of Glyphosate Mixture Containing Surfactants

다국어 초록 (Multilingual Abstract)

We investigated whether glyphosate influences the cellular toxicity of the surfactants TN-20 and LN-10 on the mouse fibroblast-like cells, alveolar epithelial cells, and a heart cell line. The cytotoxicity of TN-20 and LN-10 (0.4-100 μM), in the presence or absence of glyphosate was determined by assessing membrane integrity. TN-20 toxicity was significantly lower in the presence of 50 μM glyphosate for the fibroblast-like cell (6.25 μM; 3.9% ± 3.4% vs -4.8% ± 0.7%), for the alveolar cells (0.78 μM; 5.7% ± 0.9%vs 0.1% ± 0.6%), and for the heart cell line (25.0 μM; 7.9% ± 3.0% vs 19.4% ± 0.7%)compared to that of TN-20 alone. The cellular toxicity of LN-10 towards the fibroblast-like cells was found to be increased in the presence of 50 μM glyphosate when LN-10concentrations of 50 μM (31.3% ± 3.9% vs 19.2% ± 0.9%) and 100 μM (62.1% ± 3.4%vs 39.0% ± 0.7%) were compared to that of LN-10 alone. These results suggest that the mixture toxicity may be a factor in glyphosate-surfactant toxicity in patients with acute glyphosate herbicide intoxication.

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