생체 외 및 생체 내 실험을 통한 모무늬돌김(Pyropia seriata) 열수추출물 의 면역 증진 효과 = Immune-enhancing Effect of Aqueous Extract of Pyropia seriata In vitro and In vivo

다국어 초록 (Multilingual Abstract)

Pyropia seriata (PS), a seaweed belonging to the red algae family, is mainly cultivated in the coastal areas of Korea and Japan. PS has been reported to have functions related to heat stress tolerance; however, its other functions remain unclear. Therefore, in this study, we investigated the immune-enhancing effects of aqueous extract of PS (AePS) using mouse RAW264.7 macrophages and cyclophosphamide (CPA)-induced immuno-suppressed experimental animals. AePS significantly increased the expression of nitric oxide (NO), inducible nitiric oxide synthase, interleukin-6, and tumor necrosis factor-α without toxicity in RAW264.7 cells. Western blotting and NO assay confirmed that these effects were mediated through the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways. Daily oral administration of AePS to CPA-induced immune-suppressed animals for two weeks restored the size of their spleen to that of normal animals and caused a significant increase in splenocyte proliferation. The results of this study suggest that PS exerts an immune-enhancing effect by inducing the expression of pro-inflammatory cytokines, indicating its protential application as an immune-related functional materials.

참고문헌 (Reference)

1 Khatua S, "Understanding immune-modulatory efficacy in vitro" 352 : 109776-, 2022

2 Monmai C, "Transgenic rice seed extracts exert immunomodulatory effects by modulating immune-related biomarkers in RAW264. 7 macrophage cells" 14 : 4143-, 2022

3 Pezzanite LM, "Toll-like receptor activation of equine mesenchymal stromal cells to enhance antibacterial activity and immunomodulatory cytokine secretion" 50 : 858-871, 2021

4 Apetoh L, "Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy" 13 : 1050-1059, 2007

5 Hirayama D, "The phagocytic function of macrophage-enforcing innate immunity and tissue homeostasis" 19 : 92-, 2017

6 Han NR, "The immune-enhancing effect of anthocyanin-fucoidan nanocomplex in RAW264. 7 macrophages and cyclophophamide-induced immunosuppressed mice" 45 : e13631-, 2021

7 Cooper MD, "The evolution of adaptive immune systems" 124 : 815-822, 2006

8 Ahlmann M, "The effect of cyclophosphamide on the immune system : Implications for clinical cancer therapy" 78 : 661-671, 2016

9 Hossain A, "Sulfated polysaccharides in sea cucumbers and their biological properties : A review" 253 : 127329-, 2023

10 박욱민 ; 강동수 ; 배태진, "Studies on organic acid, vitamin and free sugar contents of commercial dried lavers(Porphyra yezoensis)cultivated in Korea" 43 : 172-177, 2014

11 Atri C, "Role of human macrophage polarization in inflammation during infectious diseases" 19 : 1801-, 2018

12 Kahn AA, "Pulling the brakes on fast and furious multiple drug-resistant(MDR)bacteria" 22 : 859-, 2021

13 Choi CW, "Pharmaceutical composition Porphyra dentata Kjellman extract for skin regeneration. KR patent 10-1981428"

14 Drazen DL, "Melatonin enhancement of splenocyte proliferation is attenuated by luzindole, a melatonin receptor antagonist" 280 : R1476-R1482, 2001

15 Martinez FO, "Macrophage activation and polarization" 13 : 453-461, 2008

16 김지회 ; 박은정, "Laver production regions recorded in the official geography books of the Joseon dynasty" 35 : 150-160, 2023

17 Gourbal B, "Innate immune memory : An evolutionary perspective" 283 : 21-40, 2018

18 Omara FO, "Immunomodulatory and protective effects of N-acetylcysteine in mitogen-activated murine splenocytes in vitro" 116 : 219-226, 1997

19 Kim HI, "Immune-enhancing effect of Sargassum horneri on cyclophosphamide-induced immunosuppression in BALB/c mice and primary cultured splenocytes" 27 : 8253-, 2022

20 Monmai C, "Immune-enhancement and anti-inflammatory activities of fatty acids extracted from Halocynthia aurantium tunic in RAW264. 7 cells" 16 : 309-, 2018

21 장고은 ; 김춘성 ; 박보람 ; 이슬아, "Immune enhancing effects of Pyropia yezoensis hydrothermal extract in RAW264. 7 cells" 15 : 33-40, 2023

22 Mansila MJ, "Hsp70 regulates immune response in experimental autoimmune encephalomyelitis" 9 : e105737-, 2014

23 Park HS, "Heat shock protein gene family of the Porphyra seriata and enhancement of heat stress tolerance by PsHSP70 in Chlamydomonas" 14 : 332-342, 2012

24 Martinex JL, "General principles of antibiotic resistance in bacteria" 11 : 33-39, 2014

25 송지혜 ; 강희범 ; 박승호 ; 정지훈 ; 박정진 ; 유양희 ; 이유현 ; 이정민 ; 김응필 ; 최경철 ; 전우진, "Extracts of Porphyra tenera(Nori seaweed)activate the immune response in mouse RAW264. 7 macrophages via NF-kappaB signaling" 20 : 1152-1159, 2017

26 Elmore SA, "Enhanced histopathology of the spleen" 34 : 648-655, 2006

27 조보미 ; 이영재 ; 박정욱 ; 박인배 ; 조정용 ; 문제학, "Constituents and antioxidant activities of lavers(Pyropia spp. )bred at the southwestern coastal area of Korea" 53 : 669-681, 2021

28 백승연 ; 김수진 ; 김다희 ; 김미리, "Comparison of quality characteristics and antioxidant activities between Porphyra yezoensis and Porphyra dentata in Korea" 48 : 1233-1243, 2019

29 Yu Y, "Biological activities and pharmaceutical applications of polysaccharide from natural resources : A review" 183 : 91-101, 2018

30 Luo M, "Antitumor and adjuvant activity of λ-carrageenan by stimulating immune response in cancer immunotherapy" 5 : 1-12, 2015

31 Liu Z, "Anti-cancer activity of porphyran and carrageenan from red seaweeds" 24 : 4286-, 2019

32 Sudeep HV, "A standardized extract of Echinacea purpurea containing higher chicoric acid content enhances immune function in murine macrophages and cyclophosphamide-induced immunosuppression mice" l61 : 1211-1221, 2023

33 Yatim KM, "A brief journey through the immune system" 10 : 1274-1281, 2015