<i>Terriglobus aquaticus</i> sp. nov., isolated from an artificial reservoir

다국어 초록 (Multilingual Abstract)

<P>A pink-pigmented, chemo-organotrophic bacterium, designated strain 03SUJ4<SUP>T</SUP>, was isolated from the freshwater of Juam reservoir, Republic of Korea (35° 03′ 43′′ N 127° 14′ 15′′ E). Cells were aerobic, Gram-reaction-negative and non-motile rods. Strain 03SUJ4<SUP>T</SUP> grew at pH 6–7 (optimum, pH 6) and at 15–30 °C (optimum, 25 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus <I>Terriglobus</I>, showing sequence similarities of 97.09 % and 96.82 % to <I>Terriglobus roseus</I> DSM 18391<SUP>T</SUP> and <I>Terriglobus saanensis</I> SP1PR4<SUP>T</SUP>, respectively. Low <I>rpoB</I> gene sequence similarity with members of the genus <I>Terriglobus</I> and different fingerprints with the repetitive primers BOX, ERIC and REP indicated that the isolate represented a novel species of the genus <I>Terriglobus</I>. The major cellular fatty acids were iso-C<SUB>15 : 0</SUB>, C<SUB>16 : 0</SUB>, C<SUB>20 : 1</SUB>ω9<I>c</I>, C<SUB>14 : 0</SUB> and summed feature 3 (C<SUB>16 : 1</SUB>ω7<I>c</I>/C<SUB>16 : 1</SUB>ω6<I>c</I>). The DNA G+C content of strain 03SUJ4<SUP>T</SUP> was 63.2±0.1 mol% (mean±<SMALL>sd</SMALL> of three determinations). The predominant menaquinone was MK-8. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. Several phenotypic characteristics served to differentiate the novel isolate from recognized members of the genus <I>Terriglobus</I>. On the basis of the evidence presented in this study, a novel species, <I>Terriglobus aquaticus</I> sp. nov. is proposed for strain 03SUJ4<SUP>T</SUP> ( = KCTC 23332<SUP>T</SUP> = JCM 17517<SUP>T</SUP>).</P>