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      항암항생재 Mitomycin C와 DNA의 상호작용 = Interaction of Antitumor, Antibiotic Mitomycin C with DNA

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      https://www.riss.kr/link?id=A19590239

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      Mitomycin C is a clinically used antitumor antibiotic that binds covalently to deoxyribonucleic under reductive or acidic catalysis. We have investigated the confirmation, the determination of thermal melting point(Tm), the inhibition of S1 unclease and exonuclease Ⅲ and the transformation of supercoiled closed circular(SCC) PBR 322 DNA on the Mitomycin C-DNA complex.
      It was identified that Mitomycin C was reduced by 0.001M HCl pH<4), 0.01M NaBH_4 and ascorbic acid. The alkylation of DNA by Mitomycin C was increased Tm; The Tm of control λDNA was 77.25℃ whereas Tm of Mitomycin C-DNA complex was 80℃. It was founded that Mitomycin C-DNA complex inhibited the activity of the S1 nuclease and exonuclease Ⅲ. The SCC pBR 322 DNA was transformed into more open circular (OC) form by increasing concentration of the Mitomycin C. It is suggested that the interaction of Mitomycin C with DNA effects the conformation of DNA.
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      Mitomycin C is a clinically used antitumor antibiotic that binds covalently to deoxyribonucleic under reductive or acidic catalysis. We have investigated the confirmation, the determination of thermal melting point(Tm), the inhibition of S1 unclease a...

      Mitomycin C is a clinically used antitumor antibiotic that binds covalently to deoxyribonucleic under reductive or acidic catalysis. We have investigated the confirmation, the determination of thermal melting point(Tm), the inhibition of S1 unclease and exonuclease Ⅲ and the transformation of supercoiled closed circular(SCC) PBR 322 DNA on the Mitomycin C-DNA complex.
      It was identified that Mitomycin C was reduced by 0.001M HCl pH<4), 0.01M NaBH_4 and ascorbic acid. The alkylation of DNA by Mitomycin C was increased Tm; The Tm of control λDNA was 77.25℃ whereas Tm of Mitomycin C-DNA complex was 80℃. It was founded that Mitomycin C-DNA complex inhibited the activity of the S1 nuclease and exonuclease Ⅲ. The SCC pBR 322 DNA was transformed into more open circular (OC) form by increasing concentration of the Mitomycin C. It is suggested that the interaction of Mitomycin C with DNA effects the conformation of DNA.

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