Drosophila auraria 自然集團의 酵素多型現像 = Enzyme Polymorphism in Natural Populations of Drosophila auraria

다국어 초록 (Multilingual Abstract)

Enzyme polymorphism in natural populations of Korean Drosophila auraria was analyzed. Electrophoretic techniques were emloyed to invesigate allelic variations at fifteen enzyme loci in eight natural populations. Eight loci (??, Me, Xdh, Odh, Adh, Ao, 6-Pgd, Fum) out of 15 loci were monomorphic with identical mobility in all populations and the other seven loci were polymorphic. Gluose-metabolizing enzymes such as ??, ??, Me, α-Gpd, 6-Pgd, Got and Fum were less genetically variable than non-glucose-metabolizeng enzymes (Acph, Aph, Adh, Est-α, Est-β, Odh, Ao, Xdh).
At the ?? locus, three alleles were detected. The ?? allele was predominant in frequency, 0.9167 or more, and its average frequency was 0.9685. A similar gene frquency pattern was observed also for the α-Gpd and Got loci in all populations. The average proportion of heterozygous individuals was 0.0600, 0.0334 and 0.0730 for the Mdh^, α-Gpd and Got loci respectively.
Six alleles were detected at the Acph locus in the present survey. The Acph⁴allele was the most frequent allele in six populations, ranging from 0.2472 to 0.4333, the averaige being 0.3350. The average proportion of heterozygous individuals at the Acph locus was 0.7684.
At the Aph locus, four alleles were detected in eight populations. The most frequent allele was Aph³, ranging from 0.2429 to 0.6304, the average being 0.3975. The average proportion of heterozygous individuals was 0.6834 for the Aph locus.
At the Est-α locus, eight alleles including a null allele were detected and the Est-α², α³,α⁴, ?? , and ?? alleles were detected in all populations. The frequency of allele ?? was considerably higher than those of the other alleles, being 0.4573 on the average. On the other hand. the α¹,α²and ?? alleles exist in low frequencies. The average proportion of heterozygous individuals for teh Est-αlocus was 0.7073.
Five alleles including a null allele were found at the Est-β locus. Among them two alleles, Est-β²and β³, were frequent alleles in most populations studied. The most frequent allele was Est-β³, ranging from 0.4231 ot 0.5455, the average being 0.4848.
The average polymorphism(%) and heterozygosity were 38.34 and 0.1395 respectively. The average genetic similarity between populations was 0.9623±0.01 and this value is as similar as other Drosophila studied. A comparison of genetic distances among eight populations estimated on the basis of the allelic frequency for fifteen enzyme loci surveyed showed little difference, giving an average distance of 0.0054±0.0025.
It is considered that the reasons of less genetic variability in the natural populations of D. auraria may be due to genetic drift.