Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution = Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution

다국어 초록 (Multilingual Abstract)

In this study, we cloned the ERF-B3 subfamily transcription factor gene BnaERF-B3-hy15 from Brassica napus L. Huyou15. This 600 bp gene encodes a 199 amino acid classic ethylene responsive factor (ERF), which shown no binding or very weak binding GCC box-binding activity by the yeast one-hybrid assay. We used gene shuffling and the yeast one-hybrid system to obtain three mutated sequences that can bind to the GCC box. Sequence analysis indicated that two residues, Gly156 in the AP2 domain and Phe62 at the N-terminal domain were mutated to arginine and serine, respectively. Changes of Gly156 to arginine and Phe62 to serine increased the GCC- binding activity of BnaERF-B3-hy15 and the alter of Gly156 to arginine changed the AP2-domain structure of BnaERF-B3- hy15. [BMB reports 2010; 43(8): 567-572]

참고문헌 (Reference)

1 Hao, D. Y., "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF Domain) in plant" 273 : 26857-26861, 1998

2 Yamaguchi-Shinozaki, K., "Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses" 57 : 781-803, 2006

3 Li, F. L., "Transcriptional profiling of imbibed Brassica napus seed" 86 : 718-730, 2005

4 Ohta, M., "Three ethhylene-responsive transcription factors in tobacco with distinct transactivation functions" 22 : 29-38, 2000

5 Liu, Y, "The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box" 580 : 1303-1308, 2006

6 Okamuro, J. K., "The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis" 7076-7081, 1997

7 Gutterson, N., "Regulation of disease resistance pathways by AP2/ERF transcription factors" 7 : 465-471, 2004

8 Stemmer, W. P. C., "Rapid evolution of a protein in vitro by DNA shuffling" 370 : 389-391, 1994

9 Chevray, P. M., "Protein interaction cloning in yeast: Identification of mammalian proteins that react with the leucine zipper of Jun" 5789-5793, 1992

10 Brannigan, J. A., "Protein engineering 20 years on" 3 : 964-970, 2002

11 Zhu, J. K., "Plant salt tolerance" 6 : 66-71, 2001

12 Zhu, J. K., "Plant salt tolerance" 6 : 66-71, 2001

13 Park, J. M., "Overexpression of the tobacco Tsi1 gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco" 13 : 1035-1046, 2001

14 Xiao-Fen Jin, "OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis" 한국생화학분자생물학회 43 (43): 34-39, 2010

15 Tamura, K., "MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0" 24 : 1596-1599, 2007

16 Morley, K. L., "Improving enzyme properties: when are closer mutations better?" 23 : 231-237, 2005

17 Zhao, T. J., "Identification of the amino acids crucial for the activities of drought responsive element binding factors (DREBs) of Brassica napus" 581 : 3044-3050, 2007

18 Xiong, A. S., "High efficiency and throughput system in directed evolution in vitro of reporter gene" 74 : 160-168, 2007

19 Zhuang, J., "Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa" 371 : 468-474, 2008

20 Grierson, D., "Gene expression in ripening tomato fruit" 3 : 113-132, 1985

21 Yang Zhang, "Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis" 한국생화학분자생물학회 42 (42): 486-492, 2009

22 Dougherty, M. J., "Directed evolution: new parts and optimized function" 20 : 486-491, 2009

23 Sakuma, Y., "DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression" 290 : 998-1009, 2002

24 Fujimoto, S. Y., "Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression" 12 : 393-404, 2000

25 Allen, M. D., "A novel mode of DNA recognition by a β-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA" 17 : 5484-5496, 1998