4염화 탄소를 이용한 SD-Rats의 진단지표와 사이토카인에 미치는 흰점박이꽃무지의 유충 추출물의 효과들 = Effects of Larval Extracts from P. brevitarsis seulensison Cytokine and Diagnostic Marker in Carbon Tetrachloride-Administered SD Rats

다국어 초록 (Multilingual Abstract)

This study was designed to determine the effects of larval extracts from Protaetia brevitarsis seulensis shade dried larva from Korea and China on liver tissues of hepatic injury groups. The experimental groups are divided into five groups; Normal, carbon tetrachloride single injection (CCl4), Pb-CHI (CCl4 + P. brevitarsis shade dried larva from China), Pb-KOR (CCl4 + P. brevitarsis shade dried larva from Korea) and SIL (CCl4 + 0.35% silymarin) groups. Sprague Dawley rats were oral injected with CCl4 at a dose l mg/kg (20% in corn oil) for induction of liver damage for 4 weeks. Each experimental group was fed with a dose 50 mg/kg of larval extracts based on medicinal preparations from 3 weeks to 4 weeks after CCl4 treatment. At the end of 4 weeks, we evaluated the serum levels of glutamate-oxaloacetate transaminase (GOT), glutamate-pyruvate transferase (GPT), alkaline phosphatase (ALP) and blood urea nitrogen (BUN) in serum and the cytokine levels of tumor necrosis factor-α) (TNF-α) and transforming growth factor-β (TGF-β) in the cells isolated from spleen and liver. The histological analysis was also conducted. The CCl4 injection reduced body weight, induced congestion of middle lobe and hepatocytic degeneration, resulting in disintegration of hepatic cords, and increased biochemical markers of blood related to hepatic injury. On the other hand, the Pb-CHI and Pb-KOR group decreased the levels of biochemical markers in blood and cytokine levels in spleen and liver. Especially, the Pb-KOR group facilitated the recovery of biochemical values of blood related to hepatic injury, hepatic lesions and fibrosis. Taken together, larval extract from P. brevitarsis might prevent acute hepatotoxicity and enhance the recovery of liver fibrosis and cirrhosis induced by CCl4, and the ingredients could be a promising candidate for the prevention and treatment of hepatic disorders.

다국어 초록 (Multilingual Abstract)

.

참고문헌 (Reference)

1 황석연, "흰쥐에서 희점박이꽃무지의 아만성독성시험" 한의병리학회 15 (15): 703-707, 2001

2 張芝利, "중국경제곤충지" 과학출판사 1984

3 古川晴男, "원색곤충백과도감" 集英社 573-574, 1977

4 윤수홍, "사염화탄소에 의해 유도된 흰쥐의 간장해에 미치는 지잠의 보호효과" 대한약학회 51 (51): 277-284, 2007

5 황석연, "사염화탄소로 유발된 랫드의 간 손상에 대한 흰점박이꽃무지(Protaetia brevitarsis) 추출물의 예방효과" 한의병리학회 19 (19): 1337-1343, 2005

6 해리슨내과학 편찬위원회, "내과학" 도서출판 정담 1610-1611, 1997

7 Xu MQ, "Zinc finger protein A20 promotes regeneration of small-forsize liver allograft and suppresses rejection and results in a longer survival in recipient rats" 152 : 35-345, 2009

8 Kreamer BL, "Use of a low-speed, iso-density percoll centrifugation method to increase the viability of solated rat hepatocyte preparations" 22 : 201-211, 1986

9 de Groot H, "The crucial role of low steady state oxygen partial pressures in haloalkane free-radical-mediated lipid peroxidation. Possible implications in haloalkane liver injury" 35 : 15-19, 1986

10 Takeda Y, "The biochemistry of animal cells, the effect of corticosteroids on leakage of enzyme from dispersed rat liver cell" 239 : 3590-3596, 1964

11 Wang B, "TGF beta-mediated upregulation of hepatic miR-181b promotes hepatocarcinogenesis by targeting TIMP3" 25 : 1787-1797, 2010

12 Pozadzides JV, "Oncogene Hepatosplenic T-cell lymphoma and TNF-α inhibitors" 2 : 611-614, 2009

13 Thenappan A, "Loss of transforming growth factor β adaptor protein β-2 spectrin leads to delayed liver regeneration in mice" 53 : 1641-1650, 2011

14 Tian Y, "Kupffer cell dependent TNF-alpha signaling mediates injury in the arterialized small-for-size liver transplantation in the mouse" 103 : 4598-3603, 2006

15 Liu XL, "Isolation and primary culture of rat hepatocytes" 1 : 77-79, 2002

16 Lee HC, "Influence of Protaetia brevitarsis extract on liver damages induced by carbon tetrachloride and ethanol in rats" 11 : 405-414, 2001

17 Li WQ, "Hepatocytic precursor (stem-like) WB-F344 cells reduce tumorigenicity of hepatoma CBRH-7919 cells via TGF-beta/Smad pathway" 23 : 1601-1607, 2010

18 Park SR, "Effect of Holotrichia diomphaliabates on carbon tertrachloride-induced hepatoxicity in rats" 4 : 27-37, 1998

19 홍승권, "B형간염바이러스 감염과 췌장암 발생의 연관성에 관한 연구" 대한간학회 16 (16): 49-56, 2010