손바닥선인장 줄기 methanol 추출물의 항균활성 = Antimicrobial Activities of Opuntia ficus-indica var. saboten Makino Methanol Extract

국문 초록 (Abstract)

손바닥선인장 추출물은 한의학에서 항염증 작용, 화상, 부종, 소화 불량 등 몇 가지의 생리학적 기능이 확인되었다. 본 연구에서는 손바닥선인장 줄기를 메탄올을 이용하여 추출한 후 그 추출물을 이용하여 항균활성을 확인한 결과 병원균 등 일반 세균에서 항균활성을 확인하였다. 특히, 약제내성균 및 혐기성 균인 여드름 원인균 뿐만 아니라 효모, 곰팡이 등 진균류에서도 항균활성을 나타내어 넓은 항균 스펙트럼을 가지고 있음을 확인할 수 있었다. 또한 열 처리, 저장온도 및 저장기간이 항균활성에 영향을 미치지 않았으므로 온도 및 기간에 안정함을 확인할 수 있었고, 식품 보존제로서의 가능성 확인 시험에서도 기존의 식품 보존제보다 안전성이 있는 항균활성 물질로 평가되었다.

다국어 초록 (Multilingual Abstract)

The Opuntia ficus-indica var. saboten Makino (Cactus) is a tropical or subtropical plant, which is cultivated or grows naturally in Jeju island. It has been widely used as folk medicine for burned wound, edema and indigestion. In addition, its extract has been claimed to have several biological activities including anti-inflammation in oriental medicine. In this study, we examined the antimicrobial activities of the methanol extract of Opuntia ficus-indica var. saboten Makino. The extract showed a broad spectrum of antimicrobial activity against pathogenic bacteria, including antibiotics resistant bacteria (MRSA, R-P. aeruginosa, VRE) and Propionibacterium acnes, yeast, and fungi. The extract retained the activity after heat treatment for 15 min at $100^{\circ}C$ and $121^{\circ}C$ and after extended storage, up to 10 weeks storage period at $4^{\circ}C$ and $25^{\circ}C$, also stably retained its activity. It showed a better inhibitoring effect to the growth of E. coli than sodium benzoate did it at the same concentration. Addition of various salts or metal ions did not affect on its antimicrobial activity. Therefore, the antimicrobial characteristics of the extract can be applicable as a natural preservative and an antimicrobial agent for bacterial disease.

참고문헌 (Reference)

1 "한국자원식물연구원" 제일출판사 1989한국식물대보감

2 "한국 식물도감 화훼류 Ⅰ" 문교부 1964

3 "광속 식물 분류학" 1994

4 "Useful plants of the world" 53 1989

5 "Stability of Betanine extracted from Opuntia ficus var" 506-510, 1996

6 "Selective inhibition of tumor necrosis factor-induced vascular cell adhesion molecular-1 gene expression by a novel flavonoid Lack of effect on transcription factor NF-kappa B" 1501-1508, 1996

7 "Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man" 152-156, 1997

8 "Peroxynitrate scavening by flavonoids" 591-593, 1997

9 "Natural antioxidants from rosemary and sage" 102-1106, 1997

10 "Methods for testing the efficacy of food antimicrobials" 1989

11 "Illustrated book of Korean Medicinal herb" 1998

12 "Effect of antioxidants on the thermostability of red pigment in pickly pear" 1013-1016, 1995

13 "Composition of Opuntia ficus-indica" 847-853, 1997

14 "Antioxidative and antimicrobial effects of water extracts with Castanea crenata leaf tea" 1128-1131, 1999

15 "Antioxidative and antimicrobial activities of Opuntia ficus indica var" 160-166, 2000

16 "Antimicrobial and antitumor activity of triterpenoids fraction from Poria cocos wolf" 1029-1033, 1999

17 "Antimicrobial activity of Ulmicortex extract" 1022-1028, 1999

18 "Antimicrobial activities of Chopi" 371-377, 1999

19 "Antibacterial activity if some essential oil components five foodborne pathogens" 2839-2845, 1995

20 "American Society for Microbiology Mannual of Clinical Microbiology" 978-987, 1985

21 "A study on antiulcer effects of Opuntia dillenii Haw on stomach ulcer induced by water-immersion stress in rat" Seoul National Univ 1997