본 연구를 통하여 청시닥나무의 에탄올 추출물은 쥐 대식 세포인 Raw264.7 세포에 LPS로 유도된 염증반응에 미치는 효과가 있음을 확인하였다. 청시닥나무 목질부와 수피부에 에탄올을 가하여 ...
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https://www.riss.kr/link?id=A60257473
2012
Korean
KCI등재,SCOPUS
학술저널
1242-1247(6쪽)
8
0
상세조회0
다운로드국문 초록 (Abstract)
본 연구를 통하여 청시닥나무의 에탄올 추출물은 쥐 대식 세포인 Raw264.7 세포에 LPS로 유도된 염증반응에 미치는 효과가 있음을 확인하였다. 청시닥나무 목질부와 수피부에 에탄올을 가하여 ...
본 연구를 통하여 청시닥나무의 에탄올 추출물은 쥐 대식 세포인 Raw264.7 세포에 LPS로 유도된 염증반응에 미치는 효과가 있음을 확인하였다. 청시닥나무 목질부와 수피부에 에탄올을 가하여 추출한 뒤 그 추출물과 분획물의 NO 생성능 및 세포증식능을 실험한 결과 수피부의 EtOAc 분획이 세포증식능에 영향을 주지 않으면서 NO의 생성을 억제함을 확인하였다. 청시닥나무 수피부 에탄올 추출물 EtOAc 분획(EFEBA)은 Raw264.7 세포에서 LPS에 의해 생성된 NO의 분비와 iNOS의 단백질 및 mRNA의 발현을 농도 의존적으로 감소시켰고, 염증 반응 시 생성되는 IL-6, IL-1β 그리고 TNF-α의 mRNA의 발현도 현저히 감소시켰다. 또한 IκBα의 degradation을 감소시키고 p65의 인산화를 감소시켜 NF-κB signaling을 통해 염증작용을 조절함을 확인하였다.
다국어 초록 (Multilingual Abstract)
Acer barbinerve Maxim belongs to the Aceraceae tree family and is often consumed as an Oriental medicine. In this study, we investigated whether or not ethanol extract from the bark of A. barbinerve Max. (EBA) inhibits lipopolysaccharide (LPS)-induced...
Acer barbinerve Maxim belongs to the Aceraceae tree family and is often consumed as an Oriental medicine. In this study, we investigated whether or not ethanol extract from the bark of A. barbinerve Max. (EBA) inhibits lipopolysaccharide (LPS)-induced inflammatory responses in Raw264.7 macrophages. EBA was fractionated using n-hexane, CH2Cl2, ethyl acetate (EtOAc), and water. Raw264.7 cells were treated with 20 μg/mL of EBA and the EBA fractions. EBA inhibited LPS-induced nitric oxide (NO) production. Among the three fractions, EtOAc fraction of EBA (EFEBA) was the most effective in inhibiting LPS-induced NO production without significant cytotoxicity in Raw264.7 cells. EFEBA futher reduced LPS-induced expression of inducible NO synthase (iNOS) proteins and its corresponding mRNA. Additionally, EFEBA decreased the mRNA levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α in LPS-treated Raw264.7 cells. Lastly, EFEBA inhibited LPS-induced degradation of the inhibitor of kappaBalpha (IκBα) as well as phosphorylation of p65 nuclear factor-κB (NF-κB). These results indicate that EFEBA exhibits strong anti-inflammatory effects and can be developed as a potential anti-inflammatory agent.
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1 Lizárraga D, "Witch hazel (Hamamelis virginiana) fractions and the importance of gallate moieties-electron transfer capacities in their antitumoral properties" 56 : 11675-11682, 2008
2 Laflamme N, "Toll-like receptor 4: the missing link of the cerebral innate immune response triggered by circulating gram-negative bacterial cell wall components" 15 : 155-116, 2001
3 Tamir S, "The role of nitric oxide(NO) in the carcinogenic process" 1288 : F31-F36, 1996
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5 Sadaki O, "The development of functional and materials" 13 : 44-50, 1996
6 Kim TW, "The Woody Plants of Korea" Kyo-Hak Publishing Co., Ltd 465-, 2010
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8 Shin NR, "Regulation of proinflammatory mediator production in RAW264.7 macrophage by Vibrio vulnificus luxS and smcR" 41 : 169-176, 2004
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주박 추출물의 아질산염 소거와 Tyrosinase, Xanthine Oxidase, ACE 저해 효과
RAW264.7 대식세포에서 왕쥐똥나무잎 추출물의 항염증 효과
골담초(Caragana sinica) 추출물의 생리활성 탐색
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2014-06-24 | 학회명변경 | 한글명 : 한국식품영양과학회지 -> 한국식품영양과학회영문명 : Journal of the Korean Society of Food Science and Nutrition -> The Korean Society of Food Science and Nutrition | |
2014-04-02 | 학회명변경 | 한글명 : 한국식품영양과학회 -> 한국식품영양과학회지영문명 : 미등록 -> Journal of the Korean Society of Food Science and Nutrition | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2005-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2002-07-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2000-01-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 1.03 | 1.03 | 1.13 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
1.18 | 1.2 | 1.993 | 0.21 |