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      高血壓 白鼠에서의 腦神經細胞膜을 通한 칼슘移動에 關한 硏究 = A Study on the Na^+-Pump and Ca^++-Transport in the Synaptosome of 1-Kidney Hypertensive Rats

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      https://www.riss.kr/link?id=A19593205

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      To investigate the change of Na-pump and Ca-transport in the synaptosome of hypertensive rats, right kidney of Sprague-Dawley rat was removed and left renal artery was cliped(external diameter, 0.38mm). After 3 to 4 weeks, We prepared synaptosome from cerebrum of normotensive and hypertensive rats, and carried out the experiments of ouabain binding, and Ca-uptake.
      In te experiment of ouabain binding, in hypertensive rats Kd was not changed but Bmax was decreased, compared to those in the normotensive rats.
      Ca-uptake in the Na-buffer was not changed, but KCI-induced Ca-uptake was increased in the both group, but more increased in the hypertensive rat.
      In the present of nifedipine, KC1-induced Ca-uptake was inhibited about 30-50% in the both group.
      From these results, it is suggested that decreased number of Na-pump and increased calcium transport via calcium channel might be important in the pathogenesis of hypertension.
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      To investigate the change of Na-pump and Ca-transport in the synaptosome of hypertensive rats, right kidney of Sprague-Dawley rat was removed and left renal artery was cliped(external diameter, 0.38mm). After 3 to 4 weeks, We prepared synaptosome from...

      To investigate the change of Na-pump and Ca-transport in the synaptosome of hypertensive rats, right kidney of Sprague-Dawley rat was removed and left renal artery was cliped(external diameter, 0.38mm). After 3 to 4 weeks, We prepared synaptosome from cerebrum of normotensive and hypertensive rats, and carried out the experiments of ouabain binding, and Ca-uptake.
      In te experiment of ouabain binding, in hypertensive rats Kd was not changed but Bmax was decreased, compared to those in the normotensive rats.
      Ca-uptake in the Na-buffer was not changed, but KCI-induced Ca-uptake was increased in the both group, but more increased in the hypertensive rat.
      In the present of nifedipine, KC1-induced Ca-uptake was inhibited about 30-50% in the both group.
      From these results, it is suggested that decreased number of Na-pump and increased calcium transport via calcium channel might be important in the pathogenesis of hypertension.

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