Study on Chitooligosaccharide Application for in vitro Tissue Culture of Sweet Potato (Ipomea batatas L.)|RISS 상세보기

다국어 초록 (Multilingual Abstract)

Study on tissue culture of sweet potato (Ipomea batatas L.) invitro and effects of chitooligosaccharide on growth of the plant were investigated. Effects of condition of sterilization and concentration of plant regulators such as: N(6) - benzyl adenine (BA), napthyl acetic acid (NAA) and kinetin (KN) on the rate of bud formation and the growth of root and shoot of the sweet potato were studied. The results showed that 20% sodium hypochlorite and 30 min for treatment was suitable for sterilization of the sweet potato tissue; the rate of contamination was only 3.3% and 76.7% bud formation; results also showed optimal concentration of BA and KN for the growth of the plantlets invitro was 2 mg/l and 1 mg/l. Effect of chitooligosaccharide supplement on the growth of sweet potato was also studied. Mugashige and Skoog (MS) medium containing BA and KN supplemented 0, 10, 20, 30 and 50 ppm chitooligosaccharide. Results showed that 20~30 ppm chitooligosaccharide was the best for growth of the root and shoot of sweet potato, number of the plantlet roots increased by 30%, the length of root also increased 40% and fresh and dry biomass of roots increased 100% compared to the control (no supplement of chitooligosaccharide). Chitooligosaccharide also increased in the number of leaf, height and fresh and dry biomass of the plantlet shoots.

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참고문헌 (Reference)

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