본 연구에서는 장기적인 자일리톨의 섭취가 Streptococcus mutans의 대표적인 독성인자 중 하나인 글루칸 생성에 미치는 영향을 알아보기 위하여 글루칸 합성효소인 glucosyltansferase의 mRNA 발현을 ...
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https://www.riss.kr/link?id=A82291632
2009
Korean
515.105
KCI등재
학술저널
531-538(8쪽)
3
0
상세조회0
다운로드국문 초록 (Abstract)
본 연구에서는 장기적인 자일리톨의 섭취가 Streptococcus mutans의 대표적인 독성인자 중 하나인 글루칸 생성에 미치는 영향을 알아보기 위하여 글루칸 합성효소인 glucosyltansferase의 mRNA 발현을 ...
본 연구에서는 장기적인 자일리톨의 섭취가 Streptococcus mutans의 대표적인 독성인자 중 하나인 글루칸 생성에 미치는 영향을 알아보기 위하여 글루칸 합성효소인 glucosyltansferase의 mRNA 발현을 실시간 역전사 중합효소 연쇄반응을 통해 평가하여 다음과 같은 결과를 얻었다.
1. 24개월 동안 자일리톨껌을 섭취한 군에서 타액 내 Streptococcus mutans의 colony 수는 통계적으로 유의하게 감소하였다(p<0.05).
2. 비수용성 글루칸 합성에 관여하는 유전자인 gtfB, gtfC의 발현은 자일리톨껌을 섭취한 군에서 시간이 지남에 따라 유의 하게 감소하였다(p<0.05). 특히 gtfB의 발현은 12개월과 24개월째 대조군에 비하여 통계적으로 유의하게 낮았고, gtfC의 발현은 24개월째 대조군에 비하여 통계적으로 유의하게 낮았다(p<0.05).
3. 수용성 글루칸 합성에 관여하는 유전자인 gtfD의 발현 역시 자일리톨껌을 섭취한 군에서 시간이 지남에 따라 유의하게 감소하였다(p<0.05). 또한 gtfD의 발현은 12개월과 24개월째 대조군에 비하여 통계적으로 유의하게 낮았다(p<0.05).
이상의 결과들을 종합해 보았을 때, 자일리톨의 섭취는 구강 내 Streptococcus mutans의 글루칸 합성 관련 유전자들의 발현을 억제시킴으로써 Streptococcus mutans의 수적인 감소를 가져오는 것으로 생각된다.
다국어 초록 (Multilingual Abstract)
Xylitol has the ability to reduce the adherence of Streptococcus mutans(S. mutans), which can make it easier to remove plaque, decrease acid production and inhibit dental caries. There are few reports on the effects of xylitol on the expression of the...
Xylitol has the ability to reduce the adherence of Streptococcus mutans(S. mutans), which can make it easier to remove plaque, decrease acid production and inhibit dental caries. There are few reports on the effects of xylitol on the expression of the virulence related genes in S. mutans. This study examined the inhibitory effect of chewing gum containing xylitol on glucan synthesis related gene expression of S. mutans. Participants were voluntarily recruited for a women's oral health prevention program, classified into two groups(a control and a xylitol group), and then followed for 2 years. Twenty salivary samples were randomly selected from each group. Colony count and real-time reverse transcription polymerase chain reaction were used to analyze the characteristics of S. mutans. The following results were obtained:
The S. mutans counts decreased steadily in the xylitol group over the study period(p<0.05). The expression of the virulence related genes (gtfB, gtfC and gtfD) was significantly lower in the xylitol group than in the control groups (p<0.05). In conclusion, these results suggest that chewing xylitol gum for a long period of time may reduce the expression of the genes associated with S. mutans virulence, which can result in a decrease growth of S. mutans colonies as a result.
참고문헌 (Reference)
1 한성근, "자일리톨 껌 저작에 의한 유치 우식증 예방효과 비교분석" 대한소아치과학회 31 (31): 159-168, 2004
2 박호원, "우식치아와 정상치아의 교합면에서 분리한 Streptococcus mutans의 비교" 대한소아치과학회 28 (28): 129-141, 2001
3 염정현, "gtf 유전자 발현에 대한 xylitol의 영향" 대한소아치과학회 31 (31): 304-310, 2004
4 이홍모, "Xylitol-resistant Streptococcus mutans와 xylitol-sensitive Streptococcus mutans의 세포 성질에 관한 비교연구" 대한소아치과학회 30 (30): 554-558, 2003
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6 Mattos-Graner RO, "Water-insoluble glucan synthesis by mutans streptococcal strains correlates with caries incidence in 12- to 30-month-old children" 79 : 1371-1377, 2000
7 Kuramitsu HK, "Virulence factors of mutans streptococci : Role of molecular genetics" 4 : 159-176, 1993
8 Scheinin A, "Turku sugar studies : an overview" 34 : 405-408, 1976
9 Trahan L, "Transport and phosphorylation of xylitol by a fructose phosphotransferase system in Streptococcus mutans" 19 : 53-63, 1985
10 Napimoga MH, "Transmission, diversity and virulence factors of Streptococcus mutans genotypes" 47 : 59-64, 2005
1 한성근, "자일리톨 껌 저작에 의한 유치 우식증 예방효과 비교분석" 대한소아치과학회 31 (31): 159-168, 2004
2 박호원, "우식치아와 정상치아의 교합면에서 분리한 Streptococcus mutans의 비교" 대한소아치과학회 28 (28): 129-141, 2001
3 염정현, "gtf 유전자 발현에 대한 xylitol의 영향" 대한소아치과학회 31 (31): 304-310, 2004
4 이홍모, "Xylitol-resistant Streptococcus mutans와 xylitol-sensitive Streptococcus mutans의 세포 성질에 관한 비교연구" 대한소아치과학회 30 (30): 554-558, 2003
5 Trahan L, "Xylitol : a review of its action on mutans streptococci and dental plaque-its clinical significance" 45 : 77-92, 1995
6 Mattos-Graner RO, "Water-insoluble glucan synthesis by mutans streptococcal strains correlates with caries incidence in 12- to 30-month-old children" 79 : 1371-1377, 2000
7 Kuramitsu HK, "Virulence factors of mutans streptococci : Role of molecular genetics" 4 : 159-176, 1993
8 Scheinin A, "Turku sugar studies : an overview" 34 : 405-408, 1976
9 Trahan L, "Transport and phosphorylation of xylitol by a fructose phosphotransferase system in Streptococcus mutans" 19 : 53-63, 1985
10 Napimoga MH, "Transmission, diversity and virulence factors of Streptococcus mutans genotypes" 47 : 59-64, 2005
11 M¨akinen KK, "Thirtynine-month xylitol chewing-gum programme in initially 8-year-old school children : a feasibility study focusing on mutans streptococci and lactobacilli" 58 : 41-50, 2008
12 Thaweboon S, "The effect of xylitol chewing gum on mutans streptococci in saliva and dental plaque" 35 : 1024-1027, 2004
13 Nascimento MM, "Streptococcus mutans genotypes isolated from root and coronal caries" 38 : 454-463, 2004
14 Zucca M, "Streptococcus mutans and dental caries : microbiological aspects" 83 : 108-117, 1990
15 Trahan L, "Selection for Streptococcus mutans with an altered xylitol transport capacity in chronic xylitol consumers" 66 : 982-988, 1987
16 Thorild I, "Salivary mutans streptococci an dental caries in three-year-old children after maternal exposure to chewing gums containing combinations of xylitol, sorbitol, chlorhexidine, and fluoride" 62 : 245-250, 2004
17 Yamashita Y, "Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model" 61 : 3811-3817, 1993
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19 Dibdin GH, "Physical and biochemical studies of Streptococcus mutans sediments suggest new factors linking the cariogenicity of plaque with its extracellular polysaccharide content" 67 : 890-895, 1988
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학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2027 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2021-01-01 | 평가 | 등재학술지 유지 (재인증) | ![]() |
2018-01-01 | 평가 | 등재학술지 선정 (계속평가) | ![]() |
2017-12-01 | 평가 | 등재후보로 하락 (계속평가) | ![]() |
2013-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2008-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2006-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2003-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | ![]() |
2002-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | ![]() |
2001-01-01 | 평가 | 등재후보학술지 선정 (신규평가) | ![]() |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.39 | 0.39 | 0.37 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.36 | 0.35 | 0.399 | 0.05 |