Obovatol extracted from Magnolia Obovata inhibits inflammation mediator generation and prostate carcinoma PC-3, LNCap cell growth through induction of apoptotic cell death via inactivation of NF-κB

다국어 초록 (Multilingual Abstract)

Objectives : To evaluate effect of obovatol extracted from Magnolia Obovata inhibits inflammation mediator generation and prostate carcinoma PC-3, LNCap cell growth through induction of apoptotic cell death via inactivation of NF-κB.Methods : We conducted an in vitro analysis to evaluate RAW264.7 cell and the prostate cell response to obovatol in order to determine the ability and related mechanism of this therapeutic agent from natural herb to inhibit inflammation and to suppress prostate cell growth by affecting inflammation-related gene expression, cell proliferation and apoptosis via inactivation of NF-κB and other cell survival signals.Results : We found that obovatol inhibited a pronounced down- regulation of nuclear localized protein levels of nuclear factor-κB family members. Down regulated NF-κB signals by obovatol is consistent with inflammation and cell growth inhibition. These data suggest that NF-κB signal may be significant contributor in obovatol-induced anti-inflammatory effect and human prostate cancer cell PC-3, LNCap cell death.

국문 초록 (Abstract)

연구목적 : 厚朴(Magnolia Obovata)에서 추출한 낮은 농도의 Obovatol 약침액의 RAW264.7 세포에서 LPS로 유발된 염증, TNF-α로 유발된 human Prostate carcinoma PC-3 및 LNCap 세포의 세포증식에 대한 영향과 그 기전을 살펴보고자 하였다.

실험방법 : RAW264.7 세포에서 LPS로 염증을 유발하고 낮은 농도의 Obovatol 약침액을 처리한 후 cell viability, NO 생성량, iNOS와 COX-2의 발현, NF-κB활성, 전사능력을 관찰하기 위해 MTT assay, NO determination assay, western blot analysis, EMSA, luciferase activity assay를 시행하였고, LNCap, PC-3 세포에 TNF-α로 증식을 유도하고 낮은 농도의 Obovatol 약침액을 처리한 후 cell growth, apoptosis 및 apoptosis와 연관된 NF-κB의 활성 변화를 관찰하기 위해 WST-1, Cell morphogy test, DAPI staining and TUNEL assay, EMSA, luciferase activity assay를 시행하였다.


결 과:

1. RAW264.7 세포에서 낮은 농도의 Obovatol 약침액 처리는 NF-κB의 활성 및 전사능력을 낮추고 iNOS와 COX-2의 발현과 NO 생성을 감소시켜 LPS로 유발된 염증을 억제하였다.

2. LNCap, PC-3 세포에서 낮은 농도의 Obovatol 약침액 처리는 NF-κB의 활성을 낮추어 세포자멸사을 촉진함으로써 TNF-α로 유발된 암세포의 성장을 억제하였다.


결 론 : 이상의 결과는 낮은 농도의 Obovatol 약침액이 항염 및 인간 전립선암세포주인 PC-3, LNCap에 대한 증식억제 효과가 있음을 입증한 것이며, 향후 이를 바탕으로 한 생체 연구에서의 긍정적인 결과는 Obovatol 약침액이 만성염증성 질환 및 전립선암의 예방과 치료에 대한 효과적인 치료제 개발에 초석이 될 것으로 기대된다.

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